(4S)-1-methyl-4-(6-methylhepta-1,5-dien-2-yl)cyclohexene

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch name: 1806PD02BBI9000
Purity: 94.1% (main component), according to certificate of analysis
Certificate of Analysis Date: June 30, 2021
Aggregate State at Room Temperature: Liquid
Colour: Colourless – yellowish
Theoretical Oxygen Demand: ThOD NH4: 3.288 mg oxygen per mg test item
Expiry Date: October 21, 2022
Storage Conditions at the Test Facility: At 4 +/- 4 °C, in the dark
Safety Precautions: Routine safety and hygienic procedures

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Activated sludge, microorganisms from a domestic waste water treatment plant will be supplied by a municipal sewage treatment plant (e.g. Rossdorf, Germany).

Duration of test (contact time):

60 d

Initial conc.:

41.6 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

The closed test flasks will be incubated in a climatic chamber under continuously stirring. Evolving carbon dioxide will be absorbed in an appropriate absorber. The consumption of oxygen will be determined by measuring the change of pressure in the flasks.

Preparation of Test Flasks: The amounts of test item and reference item will be directly weighed into the test flasks. No emulsifiers or solvents will be used.

Test duration: 28 days, extended to 60 days
Surrounding Type: Climatic chamber
Temperature: 22°C ± 1°C
Light Conditions: Darkness
pH Value: 7.4 ± 0.2

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

33

Sampling time:

60 d

Results with reference substance:

78% after 28 days of exposure.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Description of key information

Based on GLP-study according to OECD 301F, the test substance is considered as "not readily biodegradable" according to OECD criteria (33% after 60 days).

Key value for chemical safety assessment

Type of water:

freshwater