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EC number: 216-885-3 | CAS number: 1689-99-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 - 18 Jul, 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 122-2 (Algal Toxicity, Tiers I and II)
- Version / remarks:
- version: 1982
- Deviations:
- yes
- Remarks:
- The initial cell density was 3 000 cells/mL.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- At test initiation, samples were removed from the 500-mL volumetric flasks of the test solutions and the controls and frozen for analysis. In addition, six quality control (QC) samples were prepared and used to judge the precision andquality control maintained during the analytical process.
At test termination, test solutions from each treatment level were composited and three QC samples were prepared. - Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- A 0.5145-g portion of test item was weighed into a sterile beaker, rinsed into a 50 mL volumetric flask with acetone and diluted to 50 mL with acetone to create a primary stock solution of 10 mg/mL. A 0.05 mLvolume of this stock was transferred to a 500 mL volumetric flask and diluted to volume with sterile MBL medium to create the highest nominal test concentration of 1 mg/L. Serial dilutions of the 10mg/mL primary stock in acetone created 6 more secondary stock solutions containing 5, 2.5, 1.3, 0.63, 0.31 and 0.16 mg/mL. Equal volumes (0.05 mL) of secondary stock solution were transferred to 500 mL volumetric flasks and diluted to volume with sterile MBL medium to create nominal test concentrations of 0.5, 0.25, 0.13, 0.063, 0.031 and 0.016 mg a.i./L. A 500 mL solution was also prepared containing algal growth medium only and designated the control. A solvent control solution was prepared containing 0.05 mLof acetone diluted to 500 mLwith algal growth medium and was equivalent to the concentration of solvent present in all test solutions.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- 4-day old inoculum from the laboratory culture.
- Test type:
- static
- Water media type:
- other: Marine Laboratory Medium
- Limit test:
- no
- Total exposure duration:
- 120 h
- Hardness:
- Not specified
- Test temperature:
- 22 - 26 °C
- pH:
- 7.4 - 10.1
- Dissolved oxygen:
- Not specified
- Salinity:
- Not applicable
- Conductivity:
- 220 - 310 µmhos/cm
- Nominal and measured concentrations:
- Nominal concentrations: 0.016, 0.031, 0.063, 0.13, 0.25, 0.5 and 1.0 mg a.i./L
Mean measured concentrations: 0.016, 0.018, 0.041, 0.088, 0.18, 0.26 and 0.53 mg a.i./L - Details on test conditions:
- The flasks were impartially placed on an Orbit Shaker. The shaking rate of each shaker was set at 100 rpm. Lighting was provided continuously at an intensity of 4,000-5,000 lux at the solution surface. All glassware used in this study was silanized.
Stock cultures were grown In 125 mLglass flasks containing 50mL of medium. The flasks were covered with stainless steel caps which permitted gas exchange.
The initial cell density was 3 000 cells/mL in each of the three replicate per treatement and controls.
At each 24-hour interval cell counts were conducted on each replicate vessel using a hemacytometer and microscope. One sample was taken from each flask for counting. - Key result
- Duration:
- 120 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.22 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 120 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.009 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The present guideline study was conducted in compliance with GLP. Under the test conditions used, the EC10 and EC50 120h for P. subcapitata were 0.0087 mg a.i./L and 0.22 mg a.i./L, respectively. The NOEC was defined as 0.016 mg a.i./L.
Reference
Please refer to "overall remark/ attached background material" field for result tables.
Description of key information
From key study, the 120-h ErC50 and ErC10 for aquatic algae exposed to the present test material were 0.22 mg a.i./L and 0.0087 mg a.i./L in freshwater.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.22 mg/L
- EC10 or NOEC for freshwater algae:
- 0.009 mg/L
Additional information
In the key study (1990), the toxicity of test substance to aquatic algae was investigated in a 120-hours static test with Pseudokirchneriella subcapitata according to US EPA OPP 122-2 guideline. An initial cell density of 3.0 x 103cells/mL was achieved in each of the replicates per test concentration. Algae were exposed to mean measured test item concentrations of 0.016, 0.018, 0.041, 0.088, 0.18, 0.26 and 0.53 mg a.i./L, alongside with a control containing only test medium and solvent control. Based on the mean measured concentration 120-h ErC50 was 0.22 mg a.i./L and EC10 (120 h) was determined to be 0.0087 mg a.i./L.
Additionally, four studies on N. pelliculosa, S. costatum, A. flos-aquae and D. subspicatus were conducted with the substance. These studies are only mentioned in the endpoint summary because they were deviated from the current guideline for some parts.The 120-h EC50 for N. pelliculosa, S. costatum, A. flos-aquae were 0.043 mg a.i./L; 0.13 mg a.i./L and > 0.63 mg a.i./L, respectively. The 96 -h EC50 for D. subspicatus was 5.3 mg/L. The 120 -h EC10 for N. pelliculosa was 0.0085 mg a.i./L, the 120 -h NOEC for S. costatum, A. flos-aquae were 0.033 mg a.i./L and ≥ 0.63 mg a.i./L, respectively. The 96 -h NOEC for D. subspicatus was 0.32 mg a.i./L.
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