Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

According the OECD 222 and GLP study, the chronic toxicity of the test material (EC 953-378-2) on earthworms is NOEC = 95.3 mg a.i/kg dry soil (number of juvenile worms on Day 56).

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
95.3 mg/kg soil dw

Additional information

Long term toxicity on earthworms (OECD 222, GLP):

A study was performed to determine the effects of the test material on the reproduction and growth of the earthworm, Eisenia fetida. The study is considered valid as all validity criteria are fulfilled according TG OECD 222. The test item was mixed with the artificial soil to give an indication of the effects on the test organism following exposure in the field.


The definitive test was conducted which was comprised of a water control, solvent control, test item treatments applied at 16.3, 29.4, 52.9, 95.3, 171.5, 308.6, 555.6 and 1000 mg a.i./kg dry soil and toxic reference Ringer (carbendazim), applied at 5 mg a.i./kg dry soil.


Endpoints for adult mortality was demonstrated to be > and = 1000 mg a.i./kg dry soil respectively. The EC50 for the number of juveniles on Day 56 could not be estimated because there was not dose-response relationship and was therefore concluded to be >1000 mg a.i./kg dry soil. The NOEC for the number of juvenile worms on Day 56 was 95.3 mg a.i./kg dry soil. There were no statistically significant differences between the numbers of unemerged cocoons between test item treatment groups and the solvent control.


The study was considered valid as there was less than 10% adult mortality at four weeks and more than 30 juveniles had been produced in each water and solvent control replicate by the end of the test with the coefficient of variation of reproduction less than 30%. In addition, application of the toxic reference Ringer at 5 mg a.i./kg dry soil resulted in substantial and unequivocal toxic effects.