Benzenesulfonic acid, 4-C10-13-alkyl derivs.-, salts with amines, C16-C18 (even numbered) alkyl

Endpoint summary

Description of key information

There are four existing long term terrestrial toxicity tests available on the registered substance according to GLP and TG OECD. These results are summarised below :

- OECD 222 : NOEC - 56d = 95.3 mg a.i./kg dry soil.

- OECD 208 : NOEC - 21d = 333 mg a.i/kg dry soil.

- OECD 217 : NOEC - 28d = 320 mg a.i/kg dry soil.

- OECD 216 : NOEC - 28d > 1000 mg a.i/kg dry soil.

Additional information

Long-term toxicity data on three terrestrial trophic levels: earthworms, plants and micro-organisms are available on the test item (EC 953-378-2).

 

Long term toxicity on earthworms (OECD 222, GLP):

A study was performed to determine the effects of the test material on the reproduction and growth of the earthworm, Eisenia fetida. The study is considered valid as all validity criteria are fulfilled according TG OECD 222. The test item was mixed with the artificial soil to give an indication of the effects on the test organism following exposure in the field.

 

The definitive test was conducted which was comprised of a water control, solvent control, test item treatments applied at 16.3, 29.4, 52.9, 95.3, 171.5, 308.6, 555.6 and 1000 mg a.i./kg dry soil and toxic reference Ringer (carbendazim), applied at 5 mg a.i./kg dry soil.

 

Endpoints for adult mortality was demonstrated to be > and = 1000 mg a.i./kg dry soil respectively. The EC50 for the number of juveniles on Day 56 could not be estimated because there was not dose-response relationship and was therefore concluded to be >1000 mg a.i./kg dry soil. The NOEC for the number of juvenile worms on Day 56 was 95.3 mg a.i./kg dry soil. There were no statistically significant differences between the numbers of unemerged cocoons between test item treatment groups and the solvent control.

 

Toxicity on terrestrial plants (OECD 208, GLP):

This study was conducted to determine the phytotoxic effects of the test item (EC 953-378-2) on seedling emergence and growth of six non-target plant species, consisting of four dicotyledons (cabbage, carrot, lettuce and tomato) and two monocotyledons (ryegrass and onion), during early critical stages in their development. The study was considered valid as the validation criteria were met according TG OECD 208 and no visible phytotoxic effects were observed in the controls. The soil used was a sandy loam, sieved to remove all particles greater than 2mm.

 

The EC50 values for emergence and survival were estimated to be >1000 mg a.i./kg dry soil for all plant species. The NOEC values for effects on emergence and survival were 1000 mg a.i./kg dry soil for all plant species.

The No Observed Effect Concentration (NOEC) values for height compared against the solvent control were 1000 mg a.i./kg dry soil for carrot, lettuce, tomato, ryegrass and onion. For cabbage the NOEC value was 333.3 mg a.i./kg dry soil.

The No Observed Effect Concentration (NOEC) values for dry weight compared against the solvent control were 1000 mg a.i./kg dry soil all species.

 

Toxicity on micro-organisms (OECD 216 & 217, GLP):

This study was performed to assess the long-term effect of the test item (EC 953-378-2) after a single exposure, on the nitrogen transformation and carbon transformation activities of soil micro-organisms.

 

Soil microorganisms were exposed to the test item, at five treatment concentrations (10, 32, 100, 320 and 1000 mg/kg dry weight soil) for 28 days at temperatures of between 18 to 22 °C in the dark. The test soil was a sandy loam soil obtained from a site in Rugby, Warwickshire, England that had received no pesticides or fertilisers for 5 years prior to sampling.

 

To summarize, the lack of treatment related effects on nitrogen transformation activity in soil at each of the treatment levels prevented calculation of EC values and demonstrated the NOEC to be 1000 mg/kg dry weight soil. Whilst the NOEC level was considered to be 320 mg/kg dry weight soil in carbon transformation, it was considered that showed no long-term inhibitory effect on carbon transformation in soil at the concentrations tested in this study.