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Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

OECD 211 study was performed to assess the effect on reproduction of Daphnia magna over a 21-Day period, and NOEL of 1.0 mg/L was obtained

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 mg/L

Additional information

Daphnia magna were exposed to a Water Accommodated Fraction (WAF) of the test material over a range of nominal loading rates of 1.0, 3.2, 10 and 100 mg/l for a period of 21 days. The 21- Day EL50 (immobilization) values, based on nominal test concentrations, for the parental Daphnia generation (P1) were estimated to be greater than 100 mg/l loading rate WAF. The 21- Day EL50 (reproduction) value based on nominal loading rates was calculated to be 16 mg/l loading rate WAF. The “Lowest Observed Effect Loading rate” was considered to be 3.2 mg/l loading rate WAF on the basis that at this loading rate significantly fewer live young per adult (P<0.05) were produced when compared to the control. The “No Observed Effect Loading rate” was considered to be 1.0 mg/l loading rate WAF on the basis that at this loading rate there were no significant mortalities (immobilization) observed in the parental generation (P1) and that there were no significant differences (P>=0.05) between the control and the 1.0 mg/l loading rate WAF test group in terms of numbers of live young produced per adult by Day 21.


The majority of the concentration found in the test samples were below the limit of quantitation for the analytical method (LOQ) on several occasions, therefore, given that toxicity cannot be attributed to a single component or a mixture of components but to the test material as a whole the results were based on nominal loading rates only.