(RS)-3-Allyl-2-methyl-4-oxocyclopent-2- enyl (1R,3R)-2,2-dimethyl-3-(2-methyl prop-1-enyl)-cyclopropanecarboxylate (mixture of 2 isomers 1R trans: 1R/S only 1:3) (Esbiothrin)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Purity : 96.8%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

The test concentrations were analytically measured at the beginning of the test, after 48 h and at the test.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

The test organisms were checked for mortality after 2, 24, 48, 72 and 96 h from test initiation.

Test conditions

Test temperature:

18.4-21.3°C

pH:

7.02-7.82

Dissolved oxygen:

> 6.6 mg/l

Nominal and measured concentrations:

Five nominal concentration in a geometric series, namely 5.0, 8.9, 15.8, 28.1 and 50.0 microg/l.
Test item concentrated solution flow: 1.5 ml/min

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 5 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

5 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

8.6 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At the lowest test concentrations the test item level was below the LOQ nad/or Detection; in the remaining two upper test concentrations the test item was found to be less than 17.8% to 47.4% of the nominal value. Therefore the biological results are not referred to the nominal concentration but to the geometric mean of the measured concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of test item has been tested on zebra fish under flow-through conditions. The NOEC, LOEC and LC50 values after 96 hours, calculated based on the median of the measured exposure concentrations, were <5.0 micog/l, 5.0 ug/l and 8.8 microg/l (95% confidence range 6.7-11 microg/l) respectively.