1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

no

Principles of method if other than guideline:

The test was carried out using Pseudokirchneriella subcapitata strain CCAP 278/4. Liquid cultures of Pseudokirchneriella subcapitata were obtained from the Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland. Master cultures were maintained in the laboratory by the periodic replenishment of culture medium.

Prior to the start of the test sufficient master culture was added to approximately 100 mL volumes of culture media contained in conical flasks to give an initial cell density of approximately 103 cells/mL. The flasks were plugged with polyurethane foam stoppers and kept under constant agitation by orbital shaker (approximately 150 rpm) and constant illumination at 24 ±1 °C until the algal cell density was approximately 105 to 106 cells/mL.

A positive control test using potassium dichromate as the reference item was performed twice in a 12 month period to demonstrate satisfactory conditions of the test.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification: 1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)
Batch: 9002784195
CAS Number: 1326716-46-4
Purity: 99% (0.17% remaining water)
Physical State/Appearance: White solid
Expiry Date: 01 March 2020
Storage Conditions: Room temperature in the dark

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Pseudokirchneriella subcapitata is a freshwater unicellular alga, representative of primary producers found in natural waters and can therefore be considered as an important non-target organism in freshwater ecosystems.

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

15 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

2.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

9.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

5.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

biomass

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

< 0.29 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

biomass

Key result

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

0.29 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

biomass

Details on results:

Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.33 to 32 mg/L (97% to 104% of nominal) and the analysis at 72 hours showed measured test concentrations to range from 0.25 to 32 mg/L (77% to 100% of nominal). At the lower test concentrations a slight decline in measured test concentration was observed, due to this decline it was considered justifiable to base the results on the geometric mean measured test concentrations in order to give a “worst case” analysis of the data. The geometric mean measured test concentrations were 0.29, 0.88, 2.9, 9.3 and 32 mg/L.

Results with reference substance (positive control):

A positive control test was conducted between 16 July 2019 and 19 July 2019.
Used reference substance: potassium dichromate at concentrations of 0.25, 0.50, 1.0, 2.0 and 4.0 mg/L.
Exposure conditions and data evaluation for the positive control were similar to those in the definitive test.
Exposure of Pseudokirchneriella subcapitata (CCAP 278/4) to the reference item gave the following results:
ErC50 (0 to 72 hour) : 1.3 mg/L; 95% confidence limits 1.2 to 1.4 mg/L
EyC50 (0 to 72 hour) : 0.55 mg/L; 95% confidence limits 0.49 to 0.63 mg/L
No Observed Effect Concentration based on growth rate: 0.25 mg/L
No Observed Effect Concentration based on yield: 0.25 mg/L
Lowest Observed Effect Concentration based on growth rate: 0.50 mg/L
Lowest Observed Effect Concentration based on yield: 0.50 mg/L
The results from the positive control with potassium dichromate were within the normal ranges for this reference item*.

Validity criteria fulfilled:

yes

Conclusions:

EC50 value for inhibition of growth rate:15 mg 1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)/L
NOEC value for growth rate: 2.9 mg 1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)/L
LOEC value for growth rate: 9.3 mg 1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)/L

EC50 value for inhibition of yield: 5.2 mg 1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)/L
NOEC value for yield: < 0.29 mg 1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)/L (Remark: The No Observed Effect Concentration could not be determined as all inhibition points on the graph are above the EC10 value calculated from the line of best fit.)
LOEC value for yield: 0.29 mg 1,10-Decanediamine, 4-methylbenzenesulfonate (1:2)/L

Executive summary:

Introduction: A study was performed to assess the effect of 1,10 -decanediamine, 4 -methylbenzenesulfonate (1:2) on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" and Method C.3 of Commission Regulation (EC) No 761/2009.

Methods: Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to an aqueous solution of the test item at concentrations of 0.32, 1.0, 3.2, 10 and 32 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ±1 °C.

Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.

Results: Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.33 to 32 mg/L (97% to 104% of nominal) and the analysis at 72 hours showed measured test concentrations to range from 0.25 to 32 mg/L (77% to 100% of nominal). At the lower test concentrations a slight decline in measured test concentration was observed, due to this decline it was considered justifiable to base the results on the geometric mean measured test concentrations in order to give a “worst case” analysis of the data. The geometric mean measured test concentrations were 0.29, 0.88, 2.9, 9.3 and 32 mg/L.

Exposure of Pseudokirchneriella subcapitata to the test item gave the following results based on the geometric mean measured test concentrations:

Response Value	EC50 (mg(L)	95% Confidence Limits (mg(L)	No Observed Effect Concentration (NOEC) (mg/L)	Lowest Observed Effect Concentration (LOEC) (mg/L)
Growth Rate	15	12 - 19	2.9	9.3
Yield	5.2	4.1 - 6.6	< 0.29 *)	0.29

*) The No Observed Effect Concentration could not be determined as all inhibition points on the graph are above the EC₁₀ value calculated from the line of best fit.

 

Description of key information

Introduction: A study was performed to assess the effect of 1,10 -decanediamine, 4 -methylbenzenesulfonate (1:2) on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" and Method C.3 of Commission Regulation (EC) No 761/2009.

Methods: Following a preliminary range-finding test, Pseudokirchneriella subcapitata was exposed to an aqueous solution of the test item at concentrations of 0.32, 1.0, 3.2, 10 and 32 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ±1 °C.

Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.

Results: Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.33 to 32 mg/L (97% to 104% of nominal) and the analysis at 72 hours showed measured test concentrations to range from 0.25 to 32 mg/L (77% to 100% of nominal). At the lower test concentrations a slight decline in measured test concentration was observed, due to this decline it was considered justifiable to base the results on the geometric mean measured test concentrations in order to give a “worst case” analysis of the data. The geometric mean measured test concentrations were 0.29, 0.88, 2.9, 9.3 and 32 mg/L.

Exposure of Pseudokirchneriella subcapitata to the test item gave the following results based on the geometric mean measured test concentrations:

Response Value	EC50 (mg(L)	95% Confidence Limits (mg(L)	No Observed Effect Concentration (NOEC) (mg/L)	Lowest Observed Effect Concentration (LOEC) (mg/L)
Growth Rate	15	12 - 19	2.9	9.3
Yield	5.2	4.1 - 6.6	< 0.29 *)	0.29

*) The No Observed Effect Concentration could not be determined as all inhibition points on the graph are above the EC₁₀value calculated from the line of best fit.