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EC number: 851-742-4 | CAS number: 464207-51-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2009-03-09 to 2009-03-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study was performed according to OECD Guideline 201 with GLP certificate. All validity criteria were fulfilled and no deviation was observed.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Inspected on: 23 bis 25 August 2006 / Signed on 21 March 2007
- Specific details on test material used for the study:
- - Water solubility: Low, 840 µg/L in algal medium
- Vapour pressure: 0.04 Pa at 25°C
- Partition coefficient (log Pow): 6.57 (measured)
- Storage condition of test material: Room temperature, dry, well ventilated. Keep container closed and filled, keep away from heat sources, protect against high temperature. Do not re-use empty container.
- Storage stability: stable under normal conditions, avoid oxidizing materials - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: the concentrations of test substance were assessed by chemical analysis in the test solution at start and end of the test.
- Sampling method: At test sub-sample from each freshly prepared test solution were taken for chemical analysis. At the end pooled samples were taken from each test concentration. The samples for analysis were filled into 40 mL sample vials. The vials were filled up to the brim to prevent the loss of test substance into the headspace of the vials.
For sample preparation, aliquot samples ranging from 0.1 to 5.0 mL were pipetted into 8 mL test tubes, sample volumes less than 5 mL were filled up to 5 mL with purified water. Then 100 µL of the IS working solution and 400 µL cyclohexane were added. The mixtures were shaken thoroughly by hand and additionally for 10 min on a horizontal shaker (about 200 strokes/min). For a rapid phase separation the tubes are centrifuged at 2000 rpm for 2 minutes. The organic supernatants (250 µL aliquots) were transferred into the autosampler vials using disposal Paster pipettes, aliquots of 1µL were analysed by GC/MS.
- Sample storage conditions before analysis: no data - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 200 mg (214 µL) of test substance were given into 2 L growth medium under sterile conditions in a 2 L brown glass flask with a drain port near the bottom. The preparation was stirred at room temperature with around 300 rpm for 48 h. After 48 hours the preparation was left for around 18 hours (overnight) to enable droplets to move to the surface of the solution. Then, around 200 mL of the aqueous phase were drained off and discarded. The following 600 mL were used for the test preparation without filtration as the highest test concentration. Because adsorption of the test item on filter material cannot be excluded, it is preferred to use test solutions without filtration. The highest test concentration was diluted with growth medium using a spacing factor of 2 to prepare the other 4 test concentrations.
- Eluate: growth medium
- Differential loading: no data
- Controls: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data
The test item is a liquid of poor water solubility. Since the use of a dispersant should be avoided, test solutions were prepared in growth medium as described above to obtain the maximum dissolved concentration of the test substance. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: Pseudokirchneriella subcapitata SAG 61.81, Chlorophycea, Chlorophyta.
- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologisches Institut of the University at Göttingen, Albrecht von Haller Institut, Untere Karspüle 2, 37073 Göttingen, Germany. Catalog No 61.81.
- Age of inoculum (at test initiation): the initial cell concentration in the test cutures was 10 000 cells/mL
- Method of cultivation: Three days prior to testing a pre-culture was established in OECD growth medium to obtain exponentially growing algae for the test.
ACCLIMATION
none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- no
- Hardness:
- No data
- Test temperature:
- temperature was between 21.5°C and 22.5°C
- pH:
- The pH of the controls increased from 7.54 to 9.63 during the test.
In the test cultures the initial pH ranged between 7.57 and 7.84 and was between 8.11 and 10.1 at the end of the test. - Dissolved oxygen:
- no data
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 6.25, 12.5, 25, 50 and 100 mg/L
Geometric mean measured concentrations: 46.1, 83.1, 163, 371 and 715 µg/L.
The test substance levels decreased to concentrations between 61 and 74% of the initial values during the test. The evaluation of the effect concentrations was based on mean measured concentrations of the test item. See table 6.1.5/1 in "Any other information on results incl. tables". - Details on test conditions:
- TEST SYSTEM
- Test vessel: conical glass flasks tightly sealed with glass ground-in stoppers.
- Type (delete if not applicable): tightly sealed
- Material, size, headspace, fill volume: glass, 250 mL
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): not applicable
- Initial cells density: 10 000 cells/mL
- Control end cells density: 42 3400 cells/mL (mean)
- No. of organisms per vessel: not applicable
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): not applicable
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used: not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: All stock solutions for the OECD medium were prepared with purified water processed using an ELGA, PURELAB Ultra
- Total organic carbon: no data
- Particulate matter: no data
- Metals: no data
- Pesticides: no data
- Chlorine: no data
- Alkalinity: no data
- Ca/mg ratio: no data
- Conductivity: no data
- Culture medium different from test medium: no
- Intervals of water quality measurement: no data
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no data
- Photoperiod: no data
- Light intensity and quality: adjusted to approximately 7000 Lux
- Salinity (for marine algae): not applicable
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: cell concentrations were determined using an electronic particle counter (CASY 1 Model TT, Schärfe System, Reutlingen, Germany). The correctness of the electronic counts was checked by microscopic counting following internal standard operation procedures.
- Chlorophyll measurement: no
- Other: growth and growth inhibition were quantified from measurements of the algal biomass density (cell counts) as a function of time.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: no
- Test concentrations: control plus 6.25, 12.5, 25, 50 and 100 mg/L
- Results used to determine the conditions for the definitive study: not applicable - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 715 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL not determined
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 596 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL not determined
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 371 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Cell number:
See table 6.1.5/2 in "Any other information on results incl. tables".
Cell number (x 10^4)
- Percent inhibition of growth rate:
See table 6.1.5/3 in "Any other information on results incl. tables" - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 4.25 mg/L
- Other: none - Reported statistics and error estimates:
- The test results were statistically analysed to determine EC10 and EC50 values with 95% confidence intervals using Probit-analysis assuming log-normal distribution of the values by using the computer program ToxRat.
The NOEC and LOEC values for growth rate were determined by the Williams Multiple Sequential t-test. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 72h- growth rate ErC50 and ErC10 in Pseudokirchneriella subcapitata is above 0.715 mg test item/L and is equal to 0.596 mg test item/L (geometric mean measured concentration), respectively.
- Executive summary:
This study was performed to determine the toxicity of the test substance on the growth of the green alga Pseudokirchneriella subcapitata according to the OECD guideline 201, with GLP certificate.
Since the test item is a liquid of poor water solubility, the highest test solution was prepared with growth medium to obtain the maximum dissolved concentration of the test item.
Nominal loadings were: 6.25, 12.5, 25, 50 and 100 mg test item/L. The test item concentrations were chemically analysed at start and end of the test. At the start of the test, the measured concentrations were 0.0563, 0.102, 0.210, 0.433 and 0.840 mg test item/L. At end of the test, the measured concentrations were 0.0379, 0.0677, 0.127, 0.318 and 0.609 mg test item/L. Based on this, the test substance concentrations decreased from 61 to 74% of the initial values during the test. Therefore, the evaluation of the effect concentrations was based on geometric mean measured concentrations, corresponding to 0.0461, 0.0831, 0.163, 0.371 and 0.715 mg test item/L.
Effects on algal growth could be observed only at the highest test concentration where growth rate was significantly reduced by 15.2% compared to controls. Therefore, based on geometric mean measured concentrations, the 72h-growth rate ErC50 is above 0.715 mg test item/L and the 72h-growth rate NOEC is equal to 0.371 mg test item/L. However, the preferred long-term endpoint value is the 72h-growth rate EC10, determined at 0.596 mg test item/L.
Reference
Table 6.1.5/1: Measured concentrations
Nominal concentrations |
Measured concentrations |
|||
Test item loading (mg/L) |
Test start (µg/L) |
Test end (µg/L) |
Test end (% of initial concentrations) |
Mean measured concentration (µg/L) |
Control 6.25 12.5 25 50 100 |
< LOQ 56.3 102 210 433 840 |
< LOQ 37.9 67.7 127 318 609 |
- 67.3 66.4 60.6 73.5 72.5 |
- 46.1 83.1 163 371 715 |
Table 6.1.5/2: Cell number (x 10^4)
Test item (µg/L) |
Control |
46.1 |
83.1 |
163 |
371 |
715 |
24 h |
1.430 1.490 2.090 1.440 2.580 1.720 |
2.050 2.230 2.280 |
2.660 2.540 2.640 |
2.570 2.060 2.630 |
2.210 2.330 2.130 |
1.510 2.120 1.830 |
Mean : Std. Dev : n : CV : |
1.790 0.460 6 25.7 |
2.190 0.120 3 5.5 |
2.620 0.064 3 2.4 |
2.420 0.315 3 13.0 |
2.220 0.100 3 4.5 |
1.820 0.304 3 16.7 |
48 h |
11.820 10.640 14.320 9.280 17.910 12.960 |
13.850 13.830 14.940 |
14.100 14.400 12.770 |
12.390 12.950 13.440 |
10.4600 12.070 10.500 |
6.860 6.680 6.760 |
Mean : Std. Dev : n : CV : |
12.820 3.048 6 23.8 |
14.210 0.635 3 4.5 |
13.760 0.868 3 6.3 |
12.930 0.525 3 4.1 |
11.010 0.918 3 8.3 |
6.770 0.089 3 1.3 |
72 h |
49.100 40.160 40.860 38.910 45.270 39.760 |
47.950 39.210 36.820 |
45.870 45.060 51.730 |
51.870 43.050 39.190 |
40.860 43.010 38.000 |
22.740 24.260 23.060 |
Mean : Std. Dev : n : CV : |
42.340 3.991 6 9.4 |
41.330 5.859 3 14.2 |
47.550 3.640 3 7.7 |
44.700 6.500 3 14.5 |
40.620 2.513 3 6.2 |
23.350 0.801 3 3.4 |
Table 6.1.5/3: Percent inhibition of growth rate by the test item during the 72 hours test period
Test item, mean measured (µg/L) |
% inhibition of growth rate |
46.1 83.1 163 371 715 |
0.7 -3.1 -1.3 1.0 15.2 |
Description of key information
OECD Guideline 201, GLP, key study, validity 1:
72h-ErC50 > the highest attainable test substance concentration in the test conditions (geometric mean measured concentration: 0.715 mg/L)
72h-ErC10 = 0.596 mg/L (geometric mean measured concentration)
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 0.596 mg/L
Additional information
One key study is available to assess the toxicity of the registered substance on the growth of the green alga Pseudokirchneriella subcapitata.
This study (Fraunhofer, 2009) was performed on the registered substance, according to the OECD guideline 201 with GLP certificate.
Since the test item is a liquid of poor water solubility, the highest test solution was prepared with growth medium to obtain the maximum dissolved concentration of the test item. Nominal loadings were: 6.25, 12.5, 25, 50 and 100 mg test item/L. The test item concentrations were chemically analysed at start and end of the test. At the start of the test, the measured concentrations were 0.0563, 0.102, 0.210, 0.433 and 0.840 mg test item/L. At end of the test, the measured concentrations were 0.0379, 0.0677, 0.127, 0.318 and 0.609 mg test item/L. Based on this, the test substance concentrations decreased from 61 to 74% of the initial values during the test. Therefore, the evaluation of the effect concentrations was based on geometric mean measured concentrations, corresponding to 0.0461, 0.0831, 0.163, 0.371 and 0.715 mg test item/L.
Effects on algal growth could be observed only at the highest test concentration where growth rate was significantly reduced by 15.2% compared to controls. Therefore, based on geometric mean measured concentrations, the 72h-growth rate ErC50 is above 0.715 mg test item/L and the 72h-growth rate NOEC is equal to 0.371 mg test item/L. However, the preferred long-term endpoint value is the 72h-growth rate EC10, determined at 0.596 mg test item/L.
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