Benzenepropanal, 2-methyl-4-(2-methylpropyl)-

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 01, 2015 to September 22, 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Name (as stated in the report): GR-88-0778
Lot No: Batch 6
Aspect: Colourless liquid
Expiration date: March 29, 2017

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Duration of test (contact time):

60 d

Initial conc.:

30 mg/L

Based on:

test mat.

Reference substance:

other: sodium benzoate (Fluka, Buchs, Switzerland, Art. No. 71300, purity: min. 99.0 %)

Key result

Parameter:

% degradation (O2 consumption)

Value:

77

Sampling time:

60 d

Oxygen uptakes, as read on the Oxitop meters, are corrected to account for the small differences between actual and nominal concentrations of test and reference substances.

Degradation of sodium benzoate exceeded 40 % after 7 days and 65 % after 14 days: the activity of the inoculum was thus verified (validity criterion).

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

In the test conditions GR-88-0778 undergoes 77% biodegradation after 60 days.GR-88-0778 did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.According to the Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3, Part 1, 2006, paragraph 36, GR-88-0778 should be regarded as inherently and ultimately biodegradable according to this test.

Executive summary:

The Inherent Biodegradability of GR-88-0778 was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 302C.

In the test conditions GR-88-0778 undergoes 77% biodegradation after 60 days.

According to the Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3, Part 1, 2006, paragraph 36, GR-88-0778 should be regarded as inherently and ultimately biodegradable according to this test.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 03, 2015 to September 22, 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Name (as stated in the report): GR-88-0778
Lot No: Batch 6
Aspect: Colourless liquid
Expiration date: March 29, 2017

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

> 28 - < 60 d

Initial conc.:

30 mg/L

Based on:

test mat.

Reference substance:

other: Sodium benzoate (Fluka, Buchs, Switzerland, Art. No. 71300), purity : min. 99.0%.

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Parameter:

% degradation (O2 consumption)

Value:

> 18 - < 70

Sampling time:

60 d

Remarks on result:

other: 18% and 70% biodegradation were observed on the two replicate flasks, respectively.

Details on results:

Oxygen uptakes, as read on the Oxitop controller, are corrected to account for the small differences between actual and nominal concentrations of test and reference substances. Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).The repeatability validity criterion (not more than 20% difference between replicates) is fulfilled on day 28 of the test, key date for the interpretation of the results. Therefore, the test is considered valid.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

GR-88-0778 undergoes no biodegradation after 28 days in the test conditions. After 60 days 18% and 70% biodegradation were observed on the two replicate flasks, respectively.The oxygen consumption in the flasks containing GR-88-0778 was reduced compared to the flasks containing sludge only during some days of the test. However, in an OECD 302C inherent biodegradation study conducted in parallel at the same test concentration of 30 mg/l GR-88-0778 did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration (Study number 15-E103). GR-88-0778 was therefore considered to be non-toxic to the inoculum at test concentration (30 mg/l).GR-88-0778 should be regarded as not readily biodegradable according to this test. Note:On one of the replicate test flasks a biodegradation of 70% after 60 days was observed. Congruently, a biodegradation of 80% after 56 days was seen on one of the duplicate test flasks of the first test conducted from February 04, 2015 to April 01, 2015, at a test substance concentration of 17.5 mg/l . According to the Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3, Part 1, 2006, paragraphs 21 and 36, this observation can be interpreted as an indication of the inherent biodegradability of the test substance.

Executive summary:

The Ready Biodegradability of GR-88-0778 was determined by the Manometric Respirometry Test accoridng to the OECD guideline No.: 301F

GR-88-0778 undergoes no biodegradation after 28 days in the test conditions. After 60 days 18% and 70% biodegradation were observed on the two replicate flasks, respectively.

GR-88-0778 should be regarded as not readily biodegradableaccording to this test.

 

Note:

On one of the replicate test flasks a biodegradation of 70% after 60 days was observed. Congruently, a biodegradation of 80% after 56 days was seen on one of the duplicate test flasks of the first test conducted from February 04, 2015 to April 01, 2015, at a test substance concentration of 17.5 mg/l (see 4.4).

According to the Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3, Part 1, 2006, paragraphs 21 and 36, this observation can be interpreted as an indication of the inherent biodegradability of the test substance

Description of key information

Ready biodegradation

The Ready Biodegradability of GR-88-0778 was determined by the Manometric Respirometry Test accoridng to the OECD guideline No.: 301F

Inherent biodegradation

The Inherent Biodegradability of GR-88-0778 was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 302C.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater