(3aR,8aS,8bS)-1,3-Dibenzyl-2-oxodecahydrothieno[1',2':1,2]thieno[3,4-d]imidazol-5-ium bromide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15.-18.06.1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Similar to guideline and scientifically acceptable

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

Report: datalacking; no 02 values given, duration only 24 hours

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

(1S,2S,6R)-3,5-dibenzyl-4-oxo-8lambda4-thia-3,5-diazatricyclo[6.3.0.0^{2,6}]undecan-8-ylium bromide

EC Number:

700-349-5

Cas Number:

33719-11-8

Molecular formula:

C22H25BrN2OS

IUPAC Name:

(1S,2S,6R)-3,5-dibenzyl-4-oxo-8lambda4-thia-3,5-diazatricyclo[6.3.0.0^{2,6}]undecan-8-ylium bromide

Details on test material:

- Name of test material (as cited in study report): Thiophaniumbromide

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

28 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 8.3-45 mg/L

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 120 - < 1 000 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOELR

Effect conc.:

3.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

No mortality occurred in the control.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 24h for Thiophaniumbromide on Daphnia magna was determined to be 28 mg/L.

Executive summary:

Acute Toxicity Study in Daphnia magna with Thiophaniumbromide.

The study was performed under static test conditions. A range-finding test was followed by a limit test exposing Daphnia for a maximum of 24 hours to concentrations between 12.5 and 120 mg/l and a blank-control.

Thiophaniumbromide induced acute immobilisation of Daphnia magna at or below 120 mg/l. Hence, the EC50 24h was found to be 28 mg/L, the EC100 24h was >120 - <1000 mg/L.