Vinyl ethylene carbonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 28 to November 02, 2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch number: 10121040902
Purity: 99.92%

Analytical monitoring:

yes

Details on sampling:

During the test, the concentrations of the test chemical were analyzed in 0-hour and 48-hour fresh solution and 48-hour and 96-hour old solution.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1.1026 g test item were weighed and dissolved in 11 L of test water and then stirred for 24 hours to ensure maximum dissolution in test medium to get the stock solution of 100 mg/L. The stock solution was used for exposure and diluted to other test solutions. 0.50, 0.90, 1.60 and 2.80 L of stock solution of 100 mg/L were diluted to 5 L with test water to obtain the test solution of 10, 18, 32 and 56 mg/L.

Test organisms (species):

other: Rare Minnow (Gobiocypris rarus)

Details on test organisms:

TEST ORGANISM
- Common name: Rare Minnow (Gobiocypris rarus)
- Source: The fish were reproduced in this facility. Their parental generation was introduced from CTI Chemical Safety Evaluation Research Center (SUZHOU) CO., LTD.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 1-2 cm

ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: Twice on weekday and one time on weekend until 24 hours before the test was started
- Health during acclimation (any mortality observed): no

FEEDING DURING TEST (as applicable)
- Food type: no
- Frequency: Fish were not fed during the period of the test.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

40 to 250 mg/L (as CaCO3)

Test temperature:

23±2 oC

pH:

6.0 to 8.5

Dissolved oxygen:

Not less than 60% of air saturation value (ASV) during the test period

Nominal and measured concentrations:

Nominal concentrations: 10, 18, 32, 56 and 100 mg/L;
Measured concentrations: 3.36, 6.38, 9.68, 20.0 and 33.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass bottles, diameter: 16 cm, height: 26 cm
- Type (delete if not applicable): open / closed
- Aeration: Before usage, the water was aerated for more than 24 hours to attain dissolved oxygen saturation.
- Renewal rate of test solution (frequency/flow rate): 48 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.0509 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the water treated with Aquapro Pure Water System
- Total organic carbon: < 2 mg/L

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light: 8 hours darkness
- Light intensity: 698 - 712 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Biological observation:
Observation intervals: at 2 ± 0.5 h, 5 ± 1 h and 24 ± 2 h after the start of the exposure (day 0-1). On days 2-4 of the test, all vessels with living fish should be inspected twice per day (preferably early morning and late afternoon to best cover the 24-hour periods).

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: During the period of 96 hours, no death or any visible toxic signs were observed in control group and test group of 10 mg/L. At the test group of 100 mg/L, no visible toxic sign was observed and all fish died during the test.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

30.66 mg/L

95% CI:

21.04 - 44.69

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

During the 96-hour exposure period, no death and visible abnormalities were observed in the control group and test groups of 10, 18 and 32 mg/L. One died fish was observed in the test group of 56 mg/L and four died fish were observed in the test group of 100 mg/L.
According to the results and based on the measured concentrations of test item, the 24, 48 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was greater than 33.40 mg/L, and 72 and 96 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L, with 95% confidence limit of 21.04 – 44.69 mg/L.

Results with reference substance (positive control):

24-hour LC50: 299.86 mg/L
95% confidence limits: 241.82 mg/L ~ 371.83 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 96 hours LC50 of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L.

Executive summary:

A fish acute toxicity study of VEC was conducted with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period, according to OECD Guideline 203 under GLP.

According to the results and based on the measured concentrations of test item, the 24, 48 hours LC₅₀ of VEC to Rare Minnow (Gobiocypris rarus) was greater than 33.40 mg/L, and 72 and 96 hours LC₅₀ of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L, with 95% confidence limit of 21.04 – 44.69 mg/L.

Description of key information

The 96 hours LC₅₀ of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

30.66 mg/L

Additional information

A fish acute toxicity study of VEC was conducted with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period, according to OECD Guideline 203 under GLP.

According to the results and based on the measured concentrations of test item, the 24, 48 hours LC₅₀ of VEC to Rare Minnow (Gobiocypris rarus) was greater than 33.40 mg/L, and 72 and 96 hours LC₅₀ of VEC to Rare Minnow (Gobiocypris rarus) was 30.66 mg/L, with 95% confidence limit of 21.04 – 44.69 mg/L.