Registration Dossier

Diss Factsheets

Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

Additional information

Studies have been conducted on the biotransformation of a structural isomer, HCFO 1233zd(E) -  (1-Propene, 1-chloro-3,3,3-trifluoro-, (1E)-) (Schmidt et al., 2013). Male Sprague-Dawley rats and female albino New Zealand rabbits were exposed by inhalation to levels of 2,000, 5,000, and 10,000 ppm for 6 hours. Urine was collected for 48 hours after the end of the exposure period and urinary metabolites were identified by19F-NMR, LC-MS/MS and GC/MS.


The major metabolites identified in rat urine were 3,3,3-trifluorolactic acid (32%) and N-acetyl-S-(3,3,3-trifluoro-trans-propenyl)-L-cysteine (40%). Other metabolites included S-(3,3,3-trifluoro-trans-propenyl)-mercaptolactic acid; trifluoroacetic acid; 3,3,3-trifuoro-1,2-dihydroxypropane; and 3,3,3-trifluoropropionic acid. In rabbit urine,N-acetyl-S-(3,3,3-trifluoro-trans-propenyl)-L-cysteine (46% of the total) was identified. Other metabolites included S-(3,3,3-trifluoro-trans-propenyl)-mercaptolactic acid; trifluoroacetic acid; 3,3,3-trifluoro-1,2-dihydroxypropane, S-(3,3,3-trifluoro-trans-propenyl)-L-cysteine and 3,3,3-trifluoro-1-propanol. These metabolites suggest that HCFO 1233zd(E) is metabolised via glutathione conjugation and by oxidative metabolism by cytochrome P-450 In vitro studies were also carried out in the presence of liver microsomes from rats, rabbits and humans in the presence or absence of glutathione and/or a NADPH regenerating system. S-(3,3,3-trifluoro-trans-propenyl)-glutathione was the major metabolite in the liver microsomes when glutathione was present.


The quantified amounts of the metabolites excreted with urine in both mice and rabbits, suggest only a low extent and rate of biotransformation of HCFO 1233zd(E) (~ 0.01% of dose received in rabbits, and ~0.002% of dose received in rats); the major metabolites were excreted rapidly after the end of the exposures (t1/2< 6 h).