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EC number: 459-520-5 | CAS number: 132182-92-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Apr - 03 May 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 459-520-5
- EC Name:
- -
- Cas Number:
- 132182-92-4
- Molecular formula:
- Hill formula: C7H3F13O CAS formula: C7H3F13O
- IUPAC Name:
- 1,1,1,2,2,3,4,5,5,5-decafluoro-3-methoxy-4-(trifluoromethyl)pentane
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: PDC Lot 1, Pail 2
- Expiration date of the lot/batch: 23 Mar 2006
- Purity: 99.959%
- Synonyms: MTDID 665
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature in the dark
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test substance was quantitatively added directly to test vessels. Optimal contact between substance and medium was ensured by applying continuous aeration and stirring.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Sludge was collected from municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands.The sludge was coarsely sieved, washed and diluted with tap water. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (3.3 or 3.4 g/L of sludge, as used for the specific test). The sludge was collected one day before use. Before use the pH was checked (measured value: 8.0 or 7.9).
- Method of cultivation: Because the sludge was used one day after collection, 50 mL of synthetic sewage feed* was added to each litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use (*synthetic sewage feed: 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCI, 0.4 g CaCI2·2(H2O), 0.2 g MgSO4·7(H2O), 2.8 g K2HPO4 dissolved in 1 L RO water and filtered, final pH 7.4).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 18.6 - 20.4 °C
- pH:
- 8.1 - 8.4
- Dissolved oxygen:
- Continuous aeration throughout exposure period. Range of measured dissolved oxygen [DO] values at start of observation period were 6.8 - 8.7 mg/L
- Nominal and measured concentrations:
- Nominal concentrations 100 mg/L, 180 mg/L, 320 mg/L, 560 mg/L, or 1000 mg/L during 3-hour test. 30-min test done separately as limit test at 1000 mg/L. No analytical monitoring.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass bottles/vessels.
- Type (delete if not applicable): Open during exposure. Closed during observation.
- Material, size, headspace, fill volume: Test substance and 16 mL synthetic sewage were made up to 300 mL in RO water, and 200 mL sludge suspension was added to bring total volume to 500 mL in a 1-L bottle. Test solutions were transferred to a 300-mL bottle for oxygen measurements
- Aeration: Continuous aeration with pipette, and stirring.
- Nitrification assessed: no
- No. of vessels per concentration (replicates): One each for 3-hour test, two for 30-min limit test, one each for reference substance 3,5-dichlorophenol.
- No. of vessels per control (replicates): Three blanks.
- Biomass loading rate: 1.3 g/L suspended solids in the final test mixture.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges.
- Intervals of water quality measurement: Not specified. Water quality data not provided.
OTHER TEST CONDITIONS
- Adjustment of pH: none.
- Light intensity: not specified.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen concentration. Measuring equipment: WTW Inolab Oxi Level 2 supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test substance was not completely soluble in test medium at the loading rates initially prepared.
- Results with reference substance (positive control):
- - Results with reference substance valid?: Yes (see Tables 1, 2)
- Relevant EC50 values:
3 hour test, 12.1 mg/L
30 min test, 13.1 mg/L
Any other information on results incl. tables
Table 1, Results from the 3-hour test
Sample |
Conc. or loading rate (mg/L) |
Initial oxygen conc (mg O2/L) |
Respiration rate mg O2/(L∙h) |
% Inhibition relative to control mean |
Blank controls |
||||
C1 |
0 |
7.3 |
39.7 |
─ |
C2 |
0 |
7.9 |
35.3 |
─ |
C3 |
0 |
6.8 |
41.0 |
─ |
Mean (CV) | 38.7 (7.72%) | |||
Reference sub. |
||||
R1 |
1.0 |
7.5 |
35.3 |
6 |
R2 |
3.2 |
7.8 |
30.0 |
20 |
R3 |
10 |
8.2 |
21.6 |
42 |
R4 |
32 |
8.7 |
10.1 |
73 |
Test sub. |
||||
T1 |
100 |
7.8 |
34.5 |
10 |
T2 |
180 |
8.1 |
34.2 |
10 |
T3 |
320 |
7.8 |
35.6 |
7 |
T4 |
560 |
7.8 |
36.1 |
5 |
T5 |
1000 |
7.4 |
37.6 |
1 |
Table 2, Results from the 30-min test
Sample |
Conc. or loading rate (mg/L) |
Initial oxygen conc (mg O2/L) |
Respiration rate mg O2/(L∙h) |
% Inhibition relative to control mean |
Blank controls |
||||
C1 |
0 |
7.7 |
41.8 |
─ |
C2 |
0 |
8.0 |
42.2 |
─ |
C3 |
0 |
7.8 |
43.3 |
─ |
Reference sub. |
||||
R1 |
3.2 |
7.7 |
36.0 |
14 |
R2 |
10 |
8.1 |
23.5 |
44 |
R3 |
32 |
8.4 |
11.8 |
72 |
Test sub. |
||||
T1 |
1000 |
7.5 |
42.9 |
0 |
T2 |
1000 |
6.8 |
44.8 |
(-5)¹ |
1, stimulation of respiration v. controls. Result not considered significant.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- respiration > 20 mg O2/(L∙h) [38.7 mg O2/(L∙h)], CV <30% [7.72%]. EC50 for 3,5-DCP 2-25 mg/L (13.1 mg/L)
- Conclusions:
- HFE s-601 is not inhibitory to activated sludge respiration (3-h EC50 >1000 mg/L) (OECD TG209)
- Executive summary:
The toxicity of HFE s-601 to activated sludge organisms was assessed in an assay performed according to OECD TG209. The test solution was made by direct addition to test solutions. No inhibition was observed at the highest concentration tested after 30 minute and three hour exposures. The 30-min and 3-hour EC50s to sludge are >1000 mg/L.
The study was conducted in accord with GLP criteria and test method OECD209. It is classified as reliable without restrictions and is suitable for Risk Assessment, Classification & Labelling, and PBT Analysis.
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