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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
616-307-3
EC Number:
616-307-3
Cas Number:
76205-19-1
Molecular formula:
C9 H7 Cl N2 O
IUPAC Name:
616-307-3

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
-Test species and strain: Pseudokirchneriella subcapitata, Strain No. 61.81 SAG
- Age (on study day 0): A stock algal culture is maintained continuously at the test facility.
- Supplier: Collection of algal cultures in University of Göttingen/Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Nominal and measured concentrations:
10, 3.2, 1, 0.32 and 0.10 mg test item/L and a control
Recovery was between 93 and 102 % of nominal test concentration
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Erlenmeyer flasks of 50 mL
- Initial cells density: 5000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8
- Light intensity and quality: Average 5357 lux
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
5.33 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes