α,α,α-trifluoro-3-tolualdehyde

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Acute Toxicities of Organic Chemicals to Fathead Minnows (Pimephales promelas)

Author:

Geiger DL, Brooke LT, Call DJ

Year:

1986

Bibliographic source:

Vol. III. Center for Lake Superior Environmental Studies, University of Wisconsin-Superior.

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

2015

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: US-EPA: Acute Toxicities of Organic Chemicals to Fathead Minnows

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α-trifluoro-3-tolualdehyde

EC Number:

207-228-1

EC Name:

α,α,α-trifluoro-3-tolualdehyde

Cas Number:

454-89-7

Molecular formula:

C8H5F3O

IUPAC Name:

3-(trifluoromethyl)benzaldehyde

Test material form:

liquid

Specific details on test material used for the study:

- Chemical source: Aldrich Chernical Co.
- Purity: 97 %

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling schedule: 0, 24, 48, 72 and 96 hours

Test solutions

Vehicle:

no

Details on test solutions:

Toxicant stock: 125 mg/L; Diluted (Stirred)

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

Cultured at U.S. EPA Environmental Research Laboratory-Duluth and the University of Wisconsin-Superior campus.

- Age: 28 days
- Mean length (mm): 21.8
- SD Length (mm): 1.293
- Mean weight (g): 0.151
- SD Weight (g): 0.0232
- Loading (g/L): 2.517

Holding Conditions:
- Temperature: 25 °C in flowing water
- Lightning: 16-hr light-controlled photoperiod
- Feeding: frozen adult brine shrimp (Artemia sp.)

Only groups of fish. having healthy appearance and no history of unusual thermal exposure or abnormally high mortality rate were used for toxicant
exposure. Test fish were not fed 24 hr before or during a test.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Lake Superior water: hardness 51.9 mg CaCO3/L; alkalinity 44.0 mg CaCO3/L

Test temperature:

24.1 °C

pH:

7.08

Dissolved oxygen:

6.8

Salinity:

n.a.

Conductivity:

No data

Nominal and measured concentrations:

Nominal test concentration: 0.49, 0.75, 1.2, 1.8 and 2.7 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.76 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Confidence limit: 0.65-0.88 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.69 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Confidence limit: 0.59-0.79 mg/L

Details on results:

Recovery: 100.5 %

Any other information on results incl. tables

Sublethal observations / clinical signs:

Affected fish lost schooling behavior and swam near the tank surface. They were hyperactive and darkly colored, had increased respiration, and lost equilibrium prior to death. Tanks were changed daily to reduce bacterial growth and stabilize the concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In the test with a,a,a-trifluoro-m-tolualdehyde (CAS no. 454-89-7) with fish (Pimephales promelas) a LC50 of 0.76 mg/L (confidence limit: 0.65-0.88 mg/L) after 96 hours is obtained. The study was conducted according to US-EPA: Acute Toxicities of Organic Chemicals to Fathead Minnows.

Executive summary:

The study was conducted according to US-EPA: Acute Toxicities of Organic Chemicals to Fathead Minnows. In the test with a,a,a-trifluoro-m-tolualdehyde (CAS no. 454-89-7) with fish (Pimephales promelas) a LC50 of 0.76 mg/L (confidence limit: 0.65-0.88 mg/L) after 96 hours is obtained.