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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
Inoculum or test system:
activated sludge, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
20 g/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Preliminary study:
No data

Test performance:
No data
Key result
% degradation (CO2 evolution)
ca. 51.6
Sampling time:
28 d
Details on results:

During the test, three stages in the biodegradation of the test material are clearly visible:
- primary reactions up to day 3; these are manganese related processes.
- biodegradation phase from day 3 to day 6, i.e. oxidation of the organic part of the test material,
- nitrification of the test material from day 6 to day 28.

The given limits in days are indicative and there is probably no strict demarcation between the end of biodegradation and the start of nitrification. Both of these for a while processes occur side by side.

Biodegradation is the oxidation of organic carbon and other elements in an organic molecule.
Because the concentration of nitrites and nitrates was not determined for many test times (only for 14 and 28 days) the biodegradation of the test material in multiple cannot be calculated points. For this reason, the 10-day window principle was not applied.
Validity criteria fulfilled:
Interpretation of results:
inherently biodegradable
Substance is inherently biodegradable.

Description of key information

Substnce is inherentlyb iodegradabe.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable
Type of water:

Additional information