Titanium 2,2',2''-nitrilotrisethanolate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Justification for type of information:

Read across justification

Qualifier:

according to guideline

Guideline:

other: German Industrial Standard DIN 38412 part 9

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

pH:

tested in neutralised and non-neutralised test medium in parallel

Details on test conditions:

- algae test apparatus with quasi-continuous determination of optical density
- counting of cell numbers
- photometric determination of turbidity at pre-determined times
- test solutions tested non-neutralized and neutralize

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

512 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: neutralised

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: neutralised

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

216 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: non-neutralised

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

7.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: non-neutralised

Validity criteria fulfilled:

yes

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

RA

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: not specified

Deviations:

not specified

Principles of method if other than guideline:

not specified

GLP compliance:

not specified

Test organisms (species):

Skeletonema costatum

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

410 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

72 h ErC50 = 410 mg/L (growth rate method; nominal)

Description of key information

Testing was considered not necessary as alternatives to testing were available and they demonstrated to be sufficient to fulfil the legal requirements related to Annex VII, Section 9.1.1 of REACH. Therefore, the general rules for adaptation 1.2 of Annex XI of REACH regulation are met and applied. The results obtained from the three publications available from were in the EC50 range 410 -216 mg/l. the EC50 of 216 mg/l has been chosen as key for the CSA.

Key value for chemical safety assessment

EC50 for freshwater algae:

216 mg/L