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Registration Dossier
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EC number: 500-343-0 | CAS number: 157627-92-4 1 - 2.5 moles ethoxylated
Ecotoxicological information
Long-term toxicity to aquatic invertebrates
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- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the analgoue approach justification provided in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.27 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Parent (Fo) Survival and Reproduction (Mean Brood Size; Total # of young)
- Remarks on result:
- other:
- Remarks:
- Source, key, 68585-34-2, P&G, 1977
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.37 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Total young production
- Remarks on result:
- other: 95% CL 0.22-0.54 mg/L
- Remarks:
- Source, key, 68585-34-2, P&G, 1977
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Average brood size
- Remarks on result:
- other: 95% CL 0.23-0.60 mg/L
- Remarks:
- Source, key, 68585-34-2, P&G, 1977
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.52 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Percentage of days reproduction occurred
- Remarks on result:
- other: 95% CL 0.31-0.76 mg/L
- Remarks:
- Source, key, 68585-34-2, P&G, 1977
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 0.74 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL 0.56-0.94 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.17 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL 0.82-1.66 mg/L
- Remarks:
- Source, key, 68585-34-2, P&G, 1977
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Apr 1976 - Feb 1977
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- study pre-dates GLPs
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations on day 0, and every 3-5 days throughout the 21-day test.
- Sampling method: details not available in study report
- Sample storage conditions before analysis: Preserved with 1% formaldehyde, and stored at 5 C until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct dilution of the appropriate amount of test substance into blended well water
- Controls: Blended well water
- Evidence of undissolved material: No - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: N/A
- Source: In-house culture
- Age of parental stock: <12 h
- Feeding during test: yes
- Food type: ground trout chow/alfalfa mixture, 1000 mg/L
- Amount: 5 mg/L
- Frequency: Each diluter cycle
ACCLIMATION
- Acclimation period: Not specified
- Acclimation conditions (same as test or not): N/A
- Type and amount of food: N/A
- Feeding frequency: N/A
- Health during acclimation (any mortality observed): N/A
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Young were manually siphoned from existing culture units. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 120 +/- 18 mg/L as CaCO3
- Test temperature:
- 21 +/- 1 C
- pH:
- 7.5 +/- 0.2
- Dissolved oxygen:
- 8.9 +/- 0.4 mg/L
- Nominal and measured concentrations:
- The nominal test concentrations are not specified in the study report. Mean measured concentrations were 0, 0.13, 0.27, 0.47, 0.93, and 1.94 mg a.i./L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material: 1 liter glass beakers, 1 L test solution
- Aeration: N/A (test was flow-thru)
- Type of flow-through: Mount and Brungs proportional diluter, with modifications
- Renewal rate of test solution: 125 ml per 20 min cycle, or one chamber volume every 2.5 hr.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 daphnids/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Blended well water (a mixture of well water and deionized water)
- Total organic carbon: N/A
- Particulate matter: N/A
- Metals: N/A
- Pesticides: N/A
- Chlorine: N/A
- Alkalinity: N/A
- Ca/mg ratio: N/A
- Conductivity: N/A
- Salinity: N/A
- Culture medium different from test medium: No
- Intervals of water quality measurement: Daily
OTHER TEST CONDITIONS
- Adjustment of pH: N/A
- Photoperiod: N/A
- Light intensity: N/A
EFFECT PARAMETERS MEASURED: Mortality, 24 hr, 96 hr, 7 days, then daily to end of test. Reproduction, daily beginning day 7 or 8 to end of test.
RANGE-FINDING STUDY: no - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.27 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Parent (Fo) Survival and Reproduction (Mean Brood Size; Total # of young)
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.37 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Total young production
- Remarks on result:
- other: 95% CL 0.22-0.54 mg/L
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Average brood size
- Remarks on result:
- other: 95% CL 0.23-0.60 mg/L
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.52 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Percentage of days reproduction occurred
- Remarks on result:
- other: 95% CL 0.31-0.76 mg/L
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 0.74 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL 0.56-0.94 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.17 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL 0.82-1.66 mg/L
- Details on results:
- - Mortality of parent animals: Controls, 10%
- No. of offspring produced per day per female: N/A
- Body length and weight of parent animals: N/A
- Type and number of morphological abnormalities: N/A
- Type and number of behavioural abnormalities: N/A
- Number of males and females (parental): N/A
- Time to first brood release or time to hatch: 7 or 8 days
- Brood size: Controls, 8.05 (mean).
- Time to sexual maturity: N/A
- Type and magnitude of biochemical changes: N/A
- Effect concentrations exceeding solubility of substance in test medium: no - Reported statistics and error estimates:
- All results were calculated with a computerized probit analysis program after Finney (1964, 1971). Survival data for 96 hr and 21 days was used to calculate LC50 and EC50 values and confidence limits. All results were calculated based on mean measured concentrations of test material.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 21 day NOEC of C14-16EO2.25S (alkylethoxysulfate, AES) to Daphnia magna was 0.27 mg/L, based on measured concentrations. The NOEC was based on reproduction.
- Executive summary:
A 21 day chronic toxicity test of C14 -16EO2.25S (alkylethoxysulfate, AES) was conducted using Daphnia magna in a flow-through test. Mean measured test concentrations were 0, 0.13, 0.27, 0.47, 0.93, and 1.94 mg a.i./L. Four replicates per concentration, 5 organisms per replicate, were tested, using a modified Mount and Brungs proportional diluter. Survival was monitored at 24 hr., 96 hr., 7 days, and daily thereafter. Reproduction was monitored beginning with the production of the first brood on day 7 or 8, and daily thereafter.
The 21 -day NOEC, 0.27 mg/L, was based on both survival and reproduction.
EC50's for reproduction, and an LC50 for mortality, were also calculated and are available in the study report.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 20 - 27 Apr 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: EPA-600/489/001: Short term methods for measuring the chronic toxicity of effluents and receiving waters to freshwater organisms
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from all exposure concentrations in each stream channel on study days 1, 4 and 7
- Sampling method: 50 mL of sample water was removed from each of the replicate stream channels and combined to yield a 100 mL sample for each test concentration. Each sample was treated to three successive MBAS extractions using HPLC grade chloroform. Extracted material was combined and brought to 50 mL with fresh chloroform.
- Sample storage conditions before analysis: Not available - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test material stock solutions were prepared by diluting surfactants in distilled water. Stock mixtures of the test substance were made up in 4-L batches and placed on magnetic stirrers.
- Controls: dilution water without test substance
- Evidence of undissolved material: none - Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia dubia
- Strain/clone: Not available
- Justification for species other than prescribed by test guideline: Not applicable
- Source: Virginia Polytechnic Institute and State University In-house breeding culture
- Age of parental stock : Not available
- Feeding during test
- Food type: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Amount: not specified in study report
- Frequency: daily at each renewal
ACCLIMATION
- Acclimation period: Not available
- Acclimation conditions (same as test or not): Not available
- Type and amount of food: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Post exposure observation period:
- Not applicable
- Hardness:
- range of 75 - 120 mg/L as CaCO3
- Test temperature:
- range of 24.8 - 25.5 C
- pH:
- range of 7.06 - 7.88
- Dissolved oxygen:
- range of 6.3 - 7.0 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: Not available.
Measured concentrations: 0, 0.34, 1.10, 2.26, 4.64, 16.56 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material: Test chamber is a 35 mL disposable polystyrene cup fitted with 120-µM Nitex mesh on opposite sides
- Aeration: none (test was flow-through)
- Type of flow-through: peristaltic
- Renewal rate of test solution: 360mL/hr
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 1 daphnid/35 ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water as described by Lauth et al (1990)
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: Not available
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: range of 50 - 90 mg/L as CaCO3
- Ca/mg ratio: Not available
- Conductivity: 389 - 511 µmhos
- Salinity: Not available
- Culture medium different from test medium: No
- Intervals of water quality measurement: Days 1, 4 and 7 for each exposure concentration
OTHER TEST CONDITIONS
- Adjustment of pH: Not available
- Photoperiod: 16 hr light / 8 hr dark
- Light intensity: approximately 400 foot candles
EFFECT PARAMETERS MEASURED: mortality and number of young were assessed daily
RANGE-FINDING STUDY
- A 48 hr acute toxicity test was conducted prior to the chronic test. Results from the acute test were used to determine test concentrations for the chronic study. The 48 hr acute toxicity test is summarized in section 6.1.3. - Reference substance (positive control):
- no
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.64 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.34 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 7 d
- Dose descriptor:
- other: IC20
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- not specified
- Remarks on result:
- other: 0.58 - 1.69
- Details on results:
- C
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- One-way analysis of variance with Dunnett's test was used to determine the NOEC effect concentrations, based on reproductive responses. The IC20 was determined using the ICp (USEPA, Norberg-King, 1988) and Bruce and Versteeg methods.
- Validity criteria fulfilled:
- yes
- Remarks:
- 80% or greater survival in controls, 15 or more young per surviving female
- Conclusions:
- The 7-day NOEC of C12EO1S (alkylethoxysulfate, AES) to Ceriodaphnia dubia was <0.34 mg/L, based on reproduction (measured concentrations).
- Executive summary:
In a 7 -day chronic toxicity study, Ceriodaphnia dubia were exposed to C12EO1S (alkylethoxysulfate, AES) at measured concentrations of 0, 0.34, 1.10, 2.26, 4.64 and 16.56 mg/L under flow-through conditions. The 7 -day NOEC based on mortality and reproduction was 4.64 and <0.34 mg/L, respectively. The toxicity study is classified as acceptable and satisfies the validity criteria as stated in the study protocol.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 26 May - 04 Jun 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: EPA-600/489/001: Short term methods for measuring the chronic toxicity of effluents and receiving waters to freshwater organisms
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from all exposure concentrations in each stream channel on study days 1, 4 and 7
- Sampling method: 50 mL of sample water was removed from each of the replicate stream channels and combined to yield a 100 mL sample for each test concentration. Each sample was treated to three successive MBAS extractions using HPLC grade chloroform. Extracted material was combined and brought to 50 mL with fresh chloroform.
- Sample storage conditions before analysis: Not available - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test material stock solutions were prepared by diluting surfactants in distilled water. Stock mixtures of the test substance were made up in 4-L batches and placed on magnetic stirrers.
- Controls: dilution water without test substance
- Evidence of undissolved material: none - Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia dubia
- Strain/clone: Not available
- Justification for species other than prescribed by test guideline: Not applicable
- Source: Virginia Polytechnic Institute and State University In-house breeding culture
- Age of parental stock : Not available
- Feeding during test
- Food type: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Amount: not specified in study report
- Frequency: daily at each renewal
ACCLIMATION
- Acclimation period: Not available
- Acclimation conditions (same as test or not): Not available
- Type and amount of food: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Post exposure observation period:
- Not applicable
- Hardness:
- range of 60 - 120 mg/L as CaCO3
- Test temperature:
- range of 24.6 - 25.2 C
- pH:
- range of 7.10 - 8.01
- Dissolved oxygen:
- range of 6.2 - 7.9 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: Not available
Measured concentrations: 0, 0.11, 0.31, 0.70, 1.83, 4.88 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material: Test chamber is a 35 mL disposable polystyrene cup fitted with 120-µM Nitex mesh on opposite sides
- Aeration: none (test was flow-through)
- Type of flow-through: peristaltic
- Renewal rate of test solution: 360mL/hr
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 1 daphnid/35 ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water as described by Lauth et al (1990)
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: Not available
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: range of 50 - 90 mg/L as CaCO3
- Ca/mg ratio: Not available
- Conductivity: 389 - 511 µmhos
- Salinity: Not available
- Culture medium different from test medium: No
- Intervals of water quality measurement: Days 1, 4 and 7 for each exposure concentration
OTHER TEST CONDITIONS
- Adjustment of pH: Not available
- Photoperiod: 16 hr light / 8 hr dark
- Light intensity: approximately 400 foot candles
EFFECT PARAMETERS MEASURED: mortality and number of young were assessed daily
RANGE-FINDING STUDY
- A 48 hr acute toxicity test was conducted prior to the chronic test. Results from the acute test were used to determine test concentrations for the chronic study. The 48 hr acute toxicity test is summarized in section 6.1.3. - Reference substance (positive control):
- no
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.83 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.31 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 7 d
- Dose descriptor:
- other: IC20
- Effect conc.:
- 0.45 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- not specified
- Remarks on result:
- other: 0.38 - 0.50
- Details on results:
- - Mortality of parent animals: See remarks on Results
- No. of offspring produced per day per female: See remarks on Results
- Body length and weight of parent animals: Not available
- Type and number of morphological abnormalities: Not available
- Type and number of behavioural abnormalities: Not available
- Number of males and females (parental): Not available
- Time to first brood release or time to hatch: Not available
- Egg development time: Not available
- Brood size: Not available
- Time to sexual maturity: Not available
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- One-way analysis of variance with Dunnett's test was used to determine the NOEC effect concentrations, based on reproductive responses. The IC20 was determined using the ICp (USEPA, Norberg-King, 1988) and Bruce and Versteeg methods.
- Validity criteria fulfilled:
- yes
- Remarks:
- 80% or greater survival in controls, 15 or more young per surviving female
- Conclusions:
- The 7-day NOEC of C14EO2S (alkylethoxysulfate, AES) to Ceriodaphnia dubia was 0.31 mg/L, based on reproduction (measured concentrations).
- Executive summary:
In a 7 -day chronic toxicity study, Ceriodaphnia dubia were exposed to C14EO2S (alkylethoxysulfate, AES) at measured concentrations of 0, 0.11, 0.31, 0.70, 1.83 and 4.88 mg/L under flow-through conditions. The 7 -day NOEC based on mortality and reproduction was 1.83 and 0.31 mg/L, respectively. The toxicity study is classified as acceptable and satisfies the validity criteria as stated in the study protocol.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 8 - 14 Aug 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: EPA-600/489/001: Short term methods for measuring the chronic toxicity of effluents and receiving waters to freshwater organisms
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from all exposure concentrations in each stream channel on study days 1, 4 and 7
- Sampling method: 50 mL of sample water was removed from each of the replicate stream channels and combined to yield a 100 mL sample for each test concentration. Each sample was treated to three successive MBAS extractions using HPLC grade chloroform. Extracted material was combined and brought to 50 mL with fresh chloroform.
- Sample storage conditions before analysis: Not available - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test material stock solutions were prepared by diluting surfactants in distilled water. Stock mixtures of the test substance were made up in 4-L batches and placed on magnetic stirrers.
- Controls: dilution water without test substance
- Evidence of undissolved material: none - Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia dubia
- Strain/clone: Not available
- Justification for species other than prescribed by test guideline: Not applicable
- Source: Virginia Polytechnic Institute and State University In-house breeding culture
- Age of parental stock : Not available
- Feeding during test
- Food type: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Amount: not specified in study report
- Frequency: daily at each renewal
ACCLIMATION
- Acclimation period: Not available
- Acclimation conditions (same as test or not): Not available
- Type and amount of food: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Post exposure observation period:
- Not applicable
- Hardness:
- range of 75 - 120 mg/L as CaCO3
- Test temperature:
- range of 24.7 - 25.3 C
- pH:
- range of 7.30 - 7.99
- Dissolved oxygen:
- range of 6.9 - 7.3 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: Not available
Measured concentrations: 0, 0.95, 6.25, 18.50, 24.90, 34.22 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material: Test chamber is a 35 mL disposable polystyrene cup fitted with 120-µM Nitex mesh on opposite sides
- Aeration: none (test was flow-through)
- Type of flow-through: peristaltic
- Renewal rate of test solution: 360mL/hr
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 1 daphnid/35 ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water as described by Lauth et al (1990)
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: Not available
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: range of 50 - 90 mg/L as CaCO3
- Ca/mg ratio: Not available
- Conductivity: 389 - 511 µmhos
- Salinity: Not available
- Culture medium different from test medium: No
- Intervals of water quality measurement: Days 1, 4 and 7 for each exposure concentration
OTHER TEST CONDITIONS
- Adjustment of pH: Not available
- Photoperiod: 16 hr light / 8 hr dark
- Light intensity: approximately 400 foot candles
EFFECT PARAMETERS MEASURED: mortality and number of young were assessed daily
RANGE-FINDING STUDY
- A 48 hr acute toxicity test was conducted prior to the chronic test. Results from the acute test were used to determine test concentrations for the chronic study. The 48 hr acute toxicity test is summarized in section 6.1.3. - Reference substance (positive control):
- no
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 18.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 7 d
- Dose descriptor:
- other: IC20
- Effect conc.:
- 7.85 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- not specified
- Remarks on result:
- other: 4.12 - 14.80
- Details on results:
- - Mortality of parent animals: See remarks on Results
- No. of offspring produced per day per female: See remarks on Results
- Body length and weight of parent animals: Not available
- Type and number of morphological abnormalities: Not available
- Type and number of behavioural abnormalities: Not available
- Number of males and females (parental): Not available
- Time to first brood release or time to hatch: Not available
- Egg development time: Not available
- Brood size: Not available
- Time to sexual maturity: Not available
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- One-way analysis of variance with Dunnett's test was used to determine the NOEC effect concentrations, based on reproductive responses. The IC20 was determined using the ICp (USEPA, Norberg-King, 1988) and Bruce and Versteeg methods.
- Validity criteria fulfilled:
- yes
- Remarks:
- 80% or greater survival in controls, 15 or more young per surviving female
- Conclusions:
- The 7-day NOEC of C12EO2S (alkylethoxysulfate, AES) to Ceriodaphnia dubia was 6.25 mg/L, based on reproduction (measured concentrations).
- Executive summary:
In a 7 -day chronic toxicity study, Ceriodaphnia dubia were exposed to C12EO2S (alkylethoxysulfate, AES) at measured concentrations of 0, 0.95, 6.25, 18.50, 24.90 and 34.22 mg/L under flow-through conditions. The 7 -day NOEC based on mortality and reproduction was 18.50 and 6.25 mg/L, respectively. The toxicity study is classified as acceptable and satisfies the validity criteria as stated in the study protocol.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 25 - 31 May 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: EPA-600/489/001: Short term methods for measuring the chronic toxicity of effluents and receiving waters to freshwater organisms
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from all exposure concentrations in each stream channel on study days 1, 4 and 7
- Sampling method: 50 mL of sample water was removed from each of the replicate stream channels and combined to yield a 100 mL sample for each test concentration. Each sample was treated to three successive MBAS extractions using HPLC grade chloroform. Extracted material was combined and brought to 50 mL with fresh chloroform.
- Sample storage conditions before analysis: Not available - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test material stock solutions were prepared by diluting surfactants in distilled water. Stock mixtures of the test substance were made up in 4-L batches and placed on magnetic stirrers.
- Controls: dilution water without test substance
- Evidence of undissolved material: none - Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Common name: Ceriodaphnia dubia
- Strain/clone: Not available
- Justification for species other than prescribed by test guideline: Not applicable
- Source: Virginia Polytechnic Institute and State University In-house breeding culture
- Age of parental stock : Not available
- Feeding during test
- Food type: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Amount: not specified in study report
- Frequency: daily at each renewal
ACCLIMATION
- Acclimation period: Not available
- Acclimation conditions (same as test or not): Not available
- Type and amount of food: trialgal diet containing equal portions of Chlamydomonas reinhardtii, Chlamydomonas vulgaris and Ankistrodesmus falcatus
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Post exposure observation period:
- Not applicable
- Hardness:
- range of 90 - 120 mg/L as CaCO3
- Test temperature:
- range of 25.4 - 26.1 C
- pH:
- range of 7.38 - 7.89
- Dissolved oxygen:
- range of 6.3 - 7.1 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: Not available
Measured concentrations: 0, 0.04, 0.34, 0.81, 1.33, 4.51 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material: Test chamber is a 35 mL disposable polystyrene cup fitted with 120-µM Nitex mesh on opposite sides
- Aeration: none (test was flow-through)
- Type of flow-through: peristaltic
- Renewal rate of test solution: 360mL/hr
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 1 daphnid/35 ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water as described by Lauth et al (1990)
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: Not available
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: range of 50 - 90 mg/L as CaCO3
- Ca/mg ratio: Not available
- Conductivity: 389 - 511 µmhos
- Salinity: Not available
- Culture medium different from test medium: No
- Intervals of water quality measurement: Days 1, 4 and 7 for each exposure concentration
OTHER TEST CONDITIONS
- Adjustment of pH: Not available
- Photoperiod: 16 hr light / 8 hr dark
- Light intensity: approximately 400 foot candles
EFFECT PARAMETERS MEASURED: mortality and number of young were assessed daily
RANGE-FINDING STUDY
- A 48 hr acute toxicity test was conducted prior to the chronic test. Results from the acute test were used to determine test concentrations for the chronic study. The 48 hr acute toxicity test is summarized in section 6.1.3. - Reference substance (positive control):
- no
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.51 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.81 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 7 d
- Dose descriptor:
- other: IC20
- Effect conc.:
- 1.08 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- not specified
- Remarks on result:
- other: 0.93 - 1.48
- Details on results:
- - Mortality of parent animals: See remarks on Results
- No. of offspring produced per day per female: See remarks on Results
- Body length and weight of parent animals: Not available
- Type and number of morphological abnormalities: Not available
- Type and number of behavioural abnormalities: Not available
- Number of males and females (parental): Not available
- Time to first brood release or time to hatch: Not available
- Egg development time: Not available
- Brood size: Not available
- Time to sexual maturity: Not available
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- One-way analysis of variance with Dunnett's test was used to determine the NOEC effect concentrations, based on reproductive responses. The IC20 was determined using the ICp (USEPA, Norberg-King, 1988) and Bruce and Versteeg methods.
- Validity criteria fulfilled:
- yes
- Remarks:
- 80% or greater survival in controls, 15 or more young per surviving female
- Conclusions:
- The 7-day NOEC of C14EO1S (alkylethoxysulfate, AES) to Ceriodaphnia dubia was 0.81 mg/L, based on reproduction (measured concentrations).
- Executive summary:
In a 7 -day chronic toxicity study, Ceriodaphnia dubia were exposed to C14EO1S (alkylethoxysulfate, AES) at measured concentrations of 0, 0.04, 0.34, 0.81, 1.33 and 4.51 mg/L under flow-through conditions. The 7 -day NOEC based on mortality and reproduction was 4.51 and 0.81 mg/L, respectively. The toxicity study is classified as acceptable and satisfies the validity criteria as stated in the study protocol.
Referenceopen allclose all
Chronic Toxicity of Alkyl Ethoxy Sulfate (AES)* to Daphnia magna (study # 20710)
Test Concentration (mg/L) |
Mortality (%) |
Total Young Produced |
Mean Brood Size |
% of Days that Reproduction Occurred |
0 |
10 |
783 |
8.0 |
92 |
0.13 |
45 |
No data ** |
No data ** |
No data ** |
0.27 |
35 |
529 |
6.2 |
100 |
0.47 |
30 |
334 |
3.5 |
75 |
0.93 |
50 |
0 |
0 |
0 |
1.94 |
100 |
0 |
0 |
0 |
* C14-16EO2.25S.
Results are cumulative at 21 days.
Test concentration is mean measured concentration.
Statistical significance not specified in study report.
** Malfunction in feeding system caused no feed to be delivered to this test concentration.
Chronic Toxicity of C12EO1S to Ceriodaphnia dubia (study 66096)
| Neonates/Female | |||
| Actual Concentration (mg/L) | % Mortality | Mean | S.D. |
| 0 | 0 | 23.1 | 2 |
| 0.34 | 10 | 20.8 * | 2.8 |
| 1.1 | 0 | 18.5 * | 4.4 |
| 2.26 | 0 | 15.5 * | 5.4 |
| 4.64 | 0 | 11.4 * | 4.7 |
| 16.56 | 100 * | 0 * | 0 |
* Significantly different from control (0.05 level).
Chronic Toxicity of C14EO2S to Ceriodaphnia dubia (study 66096)
| Neonates/Female | |||
| Actual Concentration (mg/L) | % Mortality | Mean | S.D. |
| 0 | 10 | 25.3 | 2.6 |
| 0.11 | 10 | 26.6 | 3.3 |
| 0.31 | 10 | 26.2 | 3.8 |
| 0.70 | 0 | 13.3 * | 2.1 |
| 1.83 | 0 | 6.7 * | 4.2 |
| 4.88 | 100 * | 0 * | 0 |
* Significantly different from control (0.05 level).
Chronic Toxicity of C12EO2S to Ceriodaphnia dubia (study 66096)
| Neonates/Female | |||
| Actual Concentration (mg/L) | % Mortality | Mean | S.D. |
| 0 | 0 | 23.2 | 4.8 |
| 0.95 | 0 | 25.9 | 5.5 |
| 6.25 | 0 | 25.1 | 7.4 |
| 18.50 | 10 | 10.9 * | 5.3 |
| 24.90 | 40 | 0.6 * | 0.8 |
| 34.22 | 100 * | 0 * | 0 |
* Significantly different from control (0.05 level).
Chronic Toxicity of C14EO1S to Ceriodaphnia dubia (study 66096)
| Neonates/Female | |||
| Actual Concentration (mg/L) | % Mortality | Mean | S.D. |
| 0 | 0 | 22.6 | 2.2 |
| 0.04 | 0 | 23.2 | 1.8 |
| 0.34 | 0 | 21.2 | 2.2 |
| 0.81 | 0 | 20.4 | 1.2 |
| 1.33 | 0 | 16.4 * | 3.9 |
| 4.51 | 20 | 8.3 * | 5.7 |
* Significantly different from control (0.05 level).
Description of key information
NOEC (21 d) for Daphnia manga = 0.27 mg a.i./L (measured) related to reproduction (OECD 211)
Key value for chemical safety assessment
Additional information
The assessment is based on the data currently available. New studies, based on the category review and the final decisions issued for some of the category substances, which are also relevant for this assessment, are currently being conducted. The hazard assessment with respect to aquatic toxicity will be updated once all ongoing studies have been finalised.
No chronic invertebrate studies on toxic effects of AES(C10-16, 1-2.5 EO)MEA (CAS 157627-92-4) are available. Therefore, a read-across approach from a comparable AES(C12-14, 2.5EO)Na and some pure AES homologues (C12, 1EO; C12, 2EO; C14, 1EO and C14, 2EO)Na salts was applied.
Effects of AES (C12 -14, 2.5EO) sodium salt mixture were examined in a non-GLP study according to OECD guideline 211 (P&G, 1977). Under flow-through conditions a NOEC (21 d) of 0.27 mg a.i./L (measured) for Daphnia magna was observed. The NOEC value was based on reproduction.
Following the procedures of the EPA-guideline 600/489/00 (which is equivalent or similar to OECD 211), individuals of Ceriodaphnia dubia were exposed under flow-through conditions in separate test systems to pure AES homologue Na salts with different chain-lengths (C12 and C14) and EO concentrations (EO1 and EO2). Based on reproduction the resulting 7-day-NOECs ranged from 0.31 mg a.i./L (measured) for C14, 2EO to 6.25 mg a.i./L (measured) for C12, 2EO.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
