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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28 Oct - 03 Nov 2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION:
The test solution was prepared in the concentration 100 m/L in test medium. Five concentrations were preped by dilutions in water.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flee (D. magna strauss)
- Source: in-house culture
- Age at study initiation: 6-24h
- Method of breeding: kept in reconstituted water, water changed 3 times per week, fed with green alga
- Feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none
Hardness:
140-250 mg CaCO3/L
Test temperature:
20.0 - 20.1 °C
pH:
Control: 7.79 - at test initiation; 7.95 - at test end
Test samples: 7.42 - at test initiation; 7.52 - at test end
Dissolved oxygen:
Control: 6.18 mg/L - at test initiation; 6.64 mg/L - at test end
Test samples: 6.0 mg/L - at test initiation; 6.21 mg/L - at test end
Nominal and measured concentrations:
0, 3.21, 7.06, 15.53, 34.18, 75.19 mg/L (nominal for tested product)
Details on test conditions:
TEST SYSTEM
- Test vessel: 500 mL glass beakers
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water with additions of salts.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: not stated

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mobility after 24 and 48 h

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 0.01, 0.10, 1.0, 10, 100 mg/L
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.948 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility

Table 1. Number of immobile Daphniae at 24 and 48 hours:

Test concentration [mg/L] No. of immobile Daphniae
at 24 h at 48 h
0 0/20 0/20
3.21 5/20 11/20
7.06 5/20 12/20
15.53 5/20 19/20
34.18 10/20 20/20
75.18 19/20 20/20

Description of key information

48h EC50 (Daphnia magna, mobility) =  0.948 mg a.i./L (nominal concentration, OECD 202)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.948 mg/L

Additional information

One short-term toxicity test with aquatic invertebrates is available for C12-14 AS Zn. The GLP study (Z&S, 2014) was performed with Daphnia magna according to OECD 202 guideline. Daphnids were exposed to several test concentrations: 0, 3.21; 7.06; 15.53; 34.18; 75.19 mg product/L (nominal values). The tested product had a purity of 26.6% and the remaining was water. The TOC analysis proved that the test concentrations are stable over time. Since no other analytical measurements of the test substance were performed, the results are based on the nominal concentrations. The number of immobile organisms was evaluated after 24 and 48 hours. The 48-h EC50 value was 3.575 mg product/L and 0.948 mg a.i./L (nominal concentration).