1,3,5-Triazine-2,4,6-triamine, deammoniated

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-05-05 - 1994-06-30

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The GLP study was conducted according to an internationally accepted guideline (OECD 404). All study parameters are based on the specific guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-Triazine-2,4,6-triamine, deammoniated

EC Number:

272-034-6

EC Name:

1,3,5-Triazine-2,4,6-triamine, deammoniated

Cas Number:

68649-66-1

Molecular formula:

see structural formula below

IUPAC Name:

1,3,5-Triazine-2,4,6-triamine, deammoniated

Test animals

Species:

rabbit

Strain:

other: White russian (Albino)

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

water

Controls:

not required

Amount / concentration applied:

0.5 g

Duration of treatment / exposure:

4 hours

Observation period:

3 days

Number of animals:

2 males, 1 female

Details on study design:

One day before treatment the dorsal skin area between shoulder and sacrum was clipped free of hair. To ensure a tight contact with the skin 0.5 g of the test substance was grinded in a ball mill and moistened with 0.5 ml demineralized water and was given on the left site of the vertebral column. The site of application was covered with a patch (6.25 cm2).
The opposite area of the dorsal skin was treated in the same way but without test substance (control site). A bandage wrapped several times around the trunk (Acrylastic, No. 2408, width 8 cm; supplied by P. Beiersdorf and Co.AG, D-20245 Hamburg) provided a complete occlusion. After the end of the exposition time remaining test material was gently washed off as far as possible.
The treated skin area was assessed qualitatively (erythema/eschar resp. edema) and quantitatively (values 0 - 4) using the Draize method (1, 2, 4, 5). In addition, attention was paid to the occurrence of further reactions on the skin (e.g. corrosive effects) and to clinical symptoms (systemic toxic effects).

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 72 hours

Score:

0

Max. score:

8

Reversibility:

no data

Results 2

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

not specified

Results 3

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

not specified

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Durferrit REG 1 was tested for skin corrosive/irritating properties on the skin of rabbits. Neither erythema nor edema could be detected. REG 1 is non-irritant to the skin of rabbits.

Executive summary:

Durferrit REG 1 was tested for skin corrosive/irritating properties on the skin of rabbits. Neither erythema nor edema could be detected. REG 1 is non-irritant to the skin of rabbits.