1-chloro-4-nitrobenzene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

: only males were tested, no pathologic examination was performed, lack of details on test substance

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-nitrobenzene

EC Number:

202-809-6

EC Name:

1-chloro-4-nitrobenzene

Cas Number:

100-00-5

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 1-chloro-4-nitrobenzene
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: no data
- Weight at study initiation: 180-200 g
- Fasting period before study: no data
- Housing: 5 per cage
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

polyethylene glycol

Details on dermal exposure:

Dermal application according to the method of Noakes and Sanderson, Brit. J. Industr. Med. 26, 1969, 59
Observation time: 14 days

Duration of exposure:

24 hours

Doses:

500, 600, 700, 900, 1000, 1200 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

750 mg/kg bw

Mortality:

600 mg/kg: 2 of 10 3 days after application
700 mg/kg: 5 of 10 3-4 days after application
900 mg/kg: 8 of 10 3-4 days after application
1000 mg/kg: 8 of 10 3-4 days after application
1200 mg/kg: 10 of 10 3h-3 days after application

Clinical signs:

other: sedation, cyanotic appearance, unkempt fur, palmospasm, low body temperature

Gross pathology:

not examined

Any other information on results incl. tables

Acute dermal toxicity of p-nitrochlorobenzene in rats

Dose (mg/kg bw)	Time of death	Dead	Symptoms	Number of rats used
500 600 700 900 1000 1200	- 3d 3-4d 3-4d 3-4d 3h-2d	0 2 5 8 8 10	10 10 10 10 10 10	10 10 10 10 10 10

Value: (670 - 830 mg/kg bw)

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

Classification:
DSD: Xn, R21 Harmful in contact with skin
GHS: Acute Dermal Category 3 H311

Executive summary:

Loeser, Bayer AG, 1979

The study was performed comparable to guideline study 402 with acceptable restrictions (no pathologic examination was performed, lack of details on test substance). The acute dermal toxicity of 1-chloro-4-nitrobenzene was investigated in 10 male rats per dose according to the method of Noakes and Sanderson, 1969. The animals were dosed with 500, 600, 700, 900, 1000, 1200 mg/kg body weight. Sedation, cyanotic appearance, unkempt fur, palmospasm, low body temperature were observed at dose levels equal to and exceeding 600 mg/kg body weight. The LD50 was 750 mg/kg body weight.