1-chloro-4-nitrobenzene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: adopted according to OECD SIDS, peer reviewed data

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

Unnamed

Year:

2003

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

number of animals which show effects is missing

Principles of method if other than guideline:

Method: other: head-only exp., atmosph. contained vapor and micro-
crystalline particles (particle size: 2.7 up to greater 90 µm, respirable fraction: 6.8 to 92.3 %), 16100 mg/m³ was the highest attainable concentration

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-nitrobenzene

EC Number:

202-809-6

EC Name:

1-chloro-4-nitrobenzene

Cas Number:

100-00-5

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 1-chloro-4-nitrobenzene
- Analytical purity: 99.2 %

Test animals

Species:

rat

Strain:

other: Crl:CD

Sex:

male

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

head only

Vehicle:

other: air

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

2.63, 2.84, 3.27, 3.35, 3.73, 6.12, 9.47, 16.1 mg/l (approx. 2630, 2840, 3270, 3350, 3730,6120, 9470, 16100 mg/m³)

No. of animals per sex per dose:

10

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LCLo

Effect level:

ca. 16 100 mg/m³ air

Exp. duration:

4 h

Mortality:

at 16100 mg/m3: 1/10 3 days post exposure

Clinical signs:

other: during and immediately following exposure: cyanosis, corneal opacity, abnormal arched-back posture, lethargy, reddish-brown nasal and frothy mouth discharges,tachypnea, semi-prostration   -  from 1 - 14 days post-exposure: pallor, lacrimation, alopecia, c

Body weight:

weight loss: 6-13 % within the first 24 hours with normal gain thereafter

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

LCLo (inhalation) = 16100 mg/m3 air
Classification:
GHS: Acute Inhalation Category 4

Executive summary:

Dupont, 1981

The acute inhalative toxicity of 1-chloro-4-nitrobenzene was investigated in study comparable to OECD guideline 403 with acceptable restrictions. Rats were exposed head-only to an atmosphere containing vapour and microcrystalline particles up to 16100 mg/m³ air for 4 hours. Clinical signs of toxicity were related to dose. During and immediately after exposure cyanosis, corneal opacity, abnormal arched-back posture, lethargy, reddish-brown nasal and frothy mouth discharges, tachypnea, semi-prostration were observed. From 1 - 14 days post exposure, pallor, lacrimation, alopecia, corneal opacity, stained perineal area, dermal irritations were observed. Also weight loss of 6 - 13 % was detected within the first 24 hours but weight gain normalized therafter. Three days post exposure death occurred at 16100 mg/m³ in 1 of 10 animals. Therefore the LCLo was 16100 mg/m³ air.