trisodium ({3-[(carboxylatomethyl)amino]propyl}{3-[(carboxylatomethyl)(C16-18, C18 unsat alkyl)amino]propyl}amino)acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15-17 June 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Good quality study under GLP, however no chemical analyses. Therefore concentrations nominal.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

No Chemical analysis

Principles of method if other than guideline:

no chemical analyses

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on sampling:

no chemical analyses

Details on test solutions:

Direct dispersion in water. 200 mg of test material was dispersed in aged and dechlorinated tap water and the volume adjusted to 2 litres to give a 100 mg/L stock solution 10, 18, 32, 56, 100, 180, 320 and 560 mL aliquots of this stock solution were each separately dispersed in aged and dechlorinated tap water and the volume adjusted to 1 litre to give the test series.

The test material was suspected to adsorb to glassware and so saturation of the adsorption sites was acheived by soaking the test vessels overnight prior to the start of the test with the test solutions. At 0 hours the test vessels were emptied, rinsed with the solution to be tested and then refilled with the fresh solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquée (I.R.CH.A), France At 21 ºC in polypropylene vessels containing two litres of dechlorinated and aged tap-water. Cultures were fed daily wit mixture of fry fish food (Liquifry) and a suspension of mixed algae (predominantly Chlorella spp.). Culture conditions ensure that reproduction is by parthenogenesis.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

no

Hardness:

approximately 50 mg/L as CaCO3

Test temperature:

22ºC

pH:

7.4-7.7

Dissolved oxygen:

7.7-8.4 mg O2/L

Salinity:

no data

Nominal and measured concentrations:

nominal concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L.

Details on test conditions:

Test vessels: Glass jars each containing 200 mL test solution.
Experimental design: 9 test concentrations plus 1 control each in duplicate. 20 animals per test concentration.
Method of initiation: Daphnia were placed in the test solutions after addition of the test substance.
Loading: 20 mL test solution per organism.
Photoperiod: 16 h light : 8 h dark.
Temperature: 22 ºC
Aeration: None.
Test concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L
Medium renewal: None.
Duration of exposure: 48 hours
Criteria of effect: Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.04 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks:

30% active ingredient

Basis for effect:

mobility

Remarks on result:

other: 95%CL (1.7-2.4 mg/L)

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95%CL (5.7-8.0 mg/L)

Results with reference substance (positive control):

no reference substance tested

Reported statistics and error estimates:

48 h EC50= 6.8 mg/l with 95%CL of 5.7-8.0 mg/L

Concentration (mg/L)	Cumulative immobilized Daphnia (initial population : 10 per concentration)
	24 hours				48 hours
	R1	R2	Total	%	R1	R2	Total	%
Control	0	0	0	0	0	0	0	0
1.0	0	0	0	0	0	0	0	0
1.8	0	0	0	0	0	0	0	0
3.2	0	0	0	0	0	0	0	0
5.6	3	1	4	20	5	3	8	40
10	4	5	9	45	8	8	16	80
18	9	9	18	90	10	10	20	100
32	10	10	20	100	10	10	20	100
56	10	10	20	100	10	10	20	100
100	10	10	20	100	10	10	20	100

R1 and R2 = replicate 1 and 2

Validity criteria fulfilled:

yes

Conclusions:

Good quality study, however no chemical analyses.

Executive summary:

Study Sponsor: Berol Nobel Nacka AB

Project Number: 116/121

Test Substance: Ampholak 7 TX-T

Test Spscies: Daphnia magna (Straus) tested in the 1st instar.

Test Type: A cute toxicity. 48 h EC50 (immobilisation).

Test Conditions: Static test conditions according to OECD Guideline No. 202, Part 1, and EEC.Directive 67/548 Annex V C.2 as published.in 84/449/EEC.

Results

24 h EC50 = 9.8 mg/L 8.2 -12 mg/L 95% condident limits

48 h EC50 = 6.8 mg/L 5.7 -8.0 mg/L 95% confident limits

Time (h) 95% confidence limits (mg/l)

"no effect" level at 24 h = 3.2 mg/l

"no effect" level at 48 h = 3.2 mg/l

Description of key information

Acute daphia studies are available for Tallowamphotriamino polycarboxy glycinate (Sewell 1992) and tallowamphopolycarboxy glycinate (Douglas 1988). Both studies lack the quantification of the test substance during the test. When these studies were performed no suitable sensitive analytical method was available for the quantification of this type of test substances. The substance is however very soluble (640 g/L at pH 6.4) and is therefore expected to be completely bioavailable during the acute fish and daphnia ecotoxicity tests.

The lowest EC50 of the two studies of 2.04 mg/L based on nominal active ingredient concentration is used here as a worst-case.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.04 mg/L

Additional information

Acute daphia studies are available for Tallowamphotriamino polycarboxy glycinate (Sewell 1992) and tallowamphopolycarboxy glycinate (Douglas 1988). Both studies lack the quantification of the test substance during the test. When these studies were performed no suitable sensitive analytical method was available for the quantification of this type of test substances. The substance is however very soluble (640 g/L at pH 6.4) and is therefore expected to be completely bioavailable during the acute fish and daphnia ecotoxicity tests. During algae tests however a decrease of the dissolved test substance concentration is anticipated due to some sorption of the substance to the large surface area of the algae. Where for the acute fish and daphnia the use of nominal concentrations is considered appropriate a geometric mean should be applied to the algae test results.

 

The lowest EC50 of 2.04 mg/L based on nominal active ingredient concentration is used here as a worst-case.

The results for tallowamphopolycarboxy glycinate are in good agreement with the results observed for the strongly related substance Sodium oleylamphopolycarboxy glycinate (CAS no.: 2060541 -49 -1) for which one acute daphnia study is available and which hampers from the same short commings i.e. lack the quantification of the test substance during the test. The available acute daphnia study with the Sodium oleylamphopolycarboxy glycinate from Handley and Wetton (1992) resulted in a 48h EC50 for daphnia magna of 2.4 mg a.i./L based on nominal test concentrations.