1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl ether

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

7 - 8 April 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

not required

Vehicle:

no

Details on test solutions:

Amounts of test material (160, 280, 500, 900 and 1600 mg) were dispersed in approximately 250 mL of water and subjected to ultra sonication for approximately 30 minutes. Synthetic sewage (16 mL), activated sludge (200 mL) and water, to a final volume of 500 mL, were added to each flask to give concentrations of 320, 560, 1000, 1800 and 3200 mg/L.

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

A mixed population of activated sewage sludge micro-organisms obtained from a sewage treatment plant in Belper, Derbyshire, UK which treats predominantly domestic sewage.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

Not applicable

Hardness:

Test water - laboratory tap water - hardness was approximately 100 mg/L as CaCO3.

Test temperature:

21°C

pH:

7.2

Dissolved oxygen:

See results table

Salinity:

Not applicable

Conductivity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations were 0 (control), 320, 560, 1000, 1800 and 3200 mg/L.

Details on test conditions:

Test temperature: 21˚C.
pH: Inoculum prior to application: pH 7.2.
Values recorded in individual test vessels in results section
Aeration of dilution water: Three hours, continuous.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

1 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

2 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

The EC50 (30 minutes) was 2500 mg/L and the corresponding 3 hour value was 1500 mg/L.

Results with reference substance (positive control):

The 3,5-dichlorophenol EC50 (30 minutes) was 9.4 mg/L and the corresponding 3 hour value was 5.7 mg/L. The validity criterion for the result is that it should be between 5 and 30 mg/L, therefore the results of this study are valid.

Reported statistics and error estimates:

Graphical interpretation of the data was used.

Oxygen consumption rates and % inhibition

Concentration (mg/L)		30 minutes contact		3 hours contact
		O2consumption rates (mg O2/L/min)	% inhibition	O2consumption rates (mg O2/L/min)	% inhibition
Control	R1	0.51	-	0.44	-
	R2	0.49	-	0.53	-
Test substance	320	0.61	[22]	0.48	1
	560	0.74	[48]	0.46	5
	1000	0.54	[8]	0.28	42
	1800	0.32	36	0.17	65
	3200	0.16	68	0.07	86
3,5-ichlorophenol	3.2	0.42	16	0.33	32
	10	0.18	64	0.13	73
	32	0.10	80	0.06	88

[increase in respiration compared to the control]

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (30 minutes) was 2500 mg/L and the corresponding 3 hour value was 1500 mg/L.

Executive summary:

The toxicity to bacteria of 1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl ether was investigated according to OECD 209. Activated sludge (mixed population of different micro-organisms) was exposed for 3 hours to nominal concentrations of 320, 560, 1000, 1800 and 3200 mg/L. Two controls without test substance are included in the test design. 3,5-dichlorophenol was used as a reference substance.

Based on the results of oxygen consumption, the EC50 (3h) for 1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl etherw as estimated to be 1500 mg/L, indicating low toxicity of 1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl ether to aerobic waste water bacteria.

Description of key information

The EC50 (30 minutes) was 2500 mg/L and the corresponding 3 hour value was 1500 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

1 500 mg/L

Additional information

The toxicity to bacteria of 1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl ether was investigated according to OECD 209. Activated sludge (mixed population of different micro-organisms) was exposed for 3 hours to nominal concentrations of 320, 560, 1000, 1800 and 3200 mg/L. Two controls without test substance are included in the test design. 3,5-dichlorophenol was used as a reference substance.

Based on the results of oxygen consumption, the EC50 (3h) for 1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl ether was estimated to be 1500 mg/L, indicating low toxicity of 1,3-Propanediol, 2,2-bis(hydroxymethyl)-, allyl etherto aerobic waste water bacteria.