reaction mass of dipentarythritol and 3-mercaptopropionic acid

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Remarks:

toxicity control of biodegradation test

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-[[3-(3-mercapto-1-oxopropoxy)-2,2-bis[(3-mercapto-1-oxopropoxy)methyl]propoxy]methyl]-2-[(3-mercapto-1-oxopropoxy)methyl]propane-1,3-diyl bis[3-mercaptopropionate]

EC Number:

246-893-2

EC Name:

2-[[3-(3-mercapto-1-oxopropoxy)-2,2-bis[(3-mercapto-1-oxopropoxy)methyl]propoxy]methyl]-2-[(3-mercapto-1-oxopropoxy)methyl]propane-1,3-diyl bis[3-mercaptopropionate]

Cas Number:

25359-71-1

Molecular formula:

C28H46O13S6

IUPAC Name:

3-[(3-sulfanylpropanoyl)oxy]-2-[(3-[(3-sulfanylpropanoyl)oxy]-2,2-bis{[(3-sulfanylpropanoyl)oxy]methyl}propoxy)methyl]-2-{[(3-sulfanylpropanoyl)oxy]methyl}propyl 3-sulfanylpropanoate (non-preferred name)

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Source of inoculum/activated sludge: domestic STP east of Suzhou city (no. 20150731)
- Preparation of inoculum for exposure: sedimented sludge was resuspended in test medium 3 times
- Pretreatment: none
- Concentration of sludge: 4.0 g suspended solid/L

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

28 d

Test conditions

Details on test conditions:

TEST CONDITIONS
- Composition of medium: buffer solution with Ca, Mg, and Fe added
- Test temperature: 20+-1 °C
- pH: 7.49
- Aeration of dilution water:
- Suspended solids concentration: 30 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: weekly
- Sampling method: BOD analyzer

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes

Results and discussion

Effect concentrations

Duration:

14 d

Dose descriptor:

IC10

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

All validity criteria were fulfilled:

1) Difference between replicates was 2.9% (<20%)

2) Ref.item was degraded by 83.5% in 14 days ( min.60%)

3) Avg. O2 uptake of inoculum blank was 21.2 mg/L in 28 days (max. 60 mg/L)

4) Toxicity control showed 52.1% degradation on D14 (min. 25%)

Applicant's summary and conclusion

Conclusions:

14 d IC10 = 60 mg/L

Executive summary:

The toxicity of Di-PETMP to microorganisms was investigated during a ready biodegradation study according to OECD TG 301 F (Manometric Respirometry Test).

The test material, at a concentration of 60 mg C/L, was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in the dark at approximately 20±1°C for 28 days. Control solutions with inoculum and the standard material, sodium benzoate were used for validation purposes.

The respiration of sodium benzoate by inocculum was determined as toxicity control.

The biodegradation of the item mixture in the toxicity control was found to be 52.1% after 14 days of incubation. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 2000 mg/L, as the biodegradation in the toxicity control was higher than 25% within 14 days of incubation.