(3AS,4S,6AR)-4-METHOXYTETRAHYDROFURO[3,4- B]FURAN-2(3H)-ONE

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-01-17 to 2006-02-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identity: TIC2782 (T002907)
Batch No.: 00467090 (= charge 05C0836)
Expiration date: May 04, 2006
Purity: 99.9%
Solubility in water: 32 g/L
Aggregate state/physical form
at room temperature: Solid
Color: White
Storage conditions: At room temperature

Analytical monitoring:

yes

Details on sampling:

- Sampling method: duplicate samples were taken from the test media :
* just before the test start (day 0)
* on the last day of preparation (day 3)
* at the end of the first test medium renewal period (day 1) and at the end of the last renewal period (day 4)
- All samples were taken from the approximate center of the aquaria without mixing the test media.
- Sample storage conditions before analysis: samples were deep-frozen (~-20°C).

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test medium of the single test concentration of nominal 100 mg/L was freshly prepared by completely dissolving nominal 500 mg of the test item in 5 liters of test water using ultrasonic treatment for 10 minutes and stirring for 15 minutes at room temperature.
- Eluate: no
- Controls: yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Length at study initiation (length definition, mean, range and SD): 2.6 ± 0.1 cm
- Weight at study initiation (mean and range, SD): 0.17 ±0.03 g
- Feeding during test: no
- Food type: commercial diet fish (Tetra min Hauptfutter)

ACCLIMATION
- Acclimation period: one week prior the start to the same temperature and test water
- Acclimation conditions (same as test or not): yes, same test water and temperature
- Type and amount of food: commercial diet fish (Tetra min Hauptfutter)
- Health during acclimation (any mortality observed): no fish died and all fish were healthy

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

1250 mg CaCO3/L

Test temperature:

21-22°C

pH:

7.1-7.3

Dissolved oxygen:

7.3 - 8.6 mg/L

Salinity:

not applicable

Nominal and measured concentrations:

nominal test concentrations (mg/L) : 100
Mean measured concentration (calculated as the average over all measurements of the test concentration): 83% of the nominal value

Details on test conditions:

TEST SYSTEM
- Test vessel:glass aquarium
- Material, size, headspace, fill volume: glass aquarium with 5 liters test medium
- Aeration: test water was slightly aerated during the test period
- No. of organisms per vessel: 7
- Biomass loading rate: 0.24 g fish wet weight/L test medium.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:deionized water analytical grade salts
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Photoperiod: 16-hour light to 8-hour darkness ; 30 minute transition period
- Light intensity: 50-500 lux

TEST CONCENTRATIONS
- Spacing factor for test concentrations: no (limit test)
- Range finding study
- Test concentrations: data not shown
- Results used to determine the conditions for the definitive study: yes, range-finding test and results of a pre-experiment to determine the solubility of the test item in test water.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no remarkable observations, clear test medium
- Effect concentrations exceeding solubility of substance in test medium: no remarkable observation, clear test medium.
- 96-h NOEC >= 100 mg/L.
- The 96-hour LOEC, 96-hour LC50 and 96-hour LC100 were clearly higher than 100 mg/L. These values could not be quantified due to the absence of toxicity of T002907 at the maximum test concentration.

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of T002907 was determined in a 96-hour semi-static test. A limit test was performed to demonstrate that the test item has no toxic effect on test organisms at the nominal concentration of 100 mg/L. The mean measured test item concentration was 83% of the nominal value. The 96-hour LC50 was > 100 mg T002907/L. The results of the test can be considered reliable without restriction.

Description of key information

The study of Peither (2006), investigating the acute toxicity of T002907 in carp according to OECD guideline 203, was considered as the key study for endpoint coverage. A LC50 > 100 mg/L was obtained after an exposure of 96 hours in a semi-static test in freshwater. Based on the results it can be considered that T002907 is not harmful to fish.