1,4-butanediyl diacrylate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-butanediyl diacrylate

EC Number:

213-979-6

EC Name:

1,4-butanediyl diacrylate

Cas Number:

1070-70-8

Molecular formula:

C10H14O4

IUPAC Name:

4-(prop-2-enoyloxy)butyl prop-2-enoate

Details on test material:

- Name of test material (as cited in study report): Butandioldiacrylat
- Analytical purity: > 90 %

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: M. Gaukler, Offenbach
- Weight at study initiation: about 3 kg
- Diet: ad libitum (Ssniff K; supplying company: Intermast GmbH, Soest)
- Water: ad libitum

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 0.5 ml

Duration of treatment / exposure:

1 hour (2 animals, one male and one female) and 4 hours (2 animals both male)

Observation period:

8 days; reading:
(4 hours exposure): 4 hours, 1 day, 4 days and 8 days after the end of the exposure
(1 hour exposure): 1 hour, 1 day, 2 days, 8 days after the end of the exposure

Number of animals:

2 animals exposed for 1 hour, 2 other animals for 4 hours

Details on study design:

TEST SITE
- Area of exposure: trunk
- coverage: 2 x 2 cm

REMOVAL OF TEST SUBSTANCE
- Washing with lutrol or a water/lutrol mixture (1+1)
- Time after start of exposure: 1 and 4 hours after application

SCORING SYSTEM:
similar to the one described in 404 OECD guideline

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 4 hour exposure

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 h

Score:

4

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 4 hour exposure

Results 3

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 and 48 hours

Score:

2.5

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 1 hour exposure

Results 4

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 and 48 hours

Score:

3.25

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: 1 hour exposure

Other effects:

4h: Necrosis detected in both animals, though in one animal reduced to scaling at the end of the observation period.
1h: Scaling at the end of the obervation period. No necrosis occured.

Any other information on results incl. tables

Draize scores of individual animals:

1 hour exposure	time	animal 1	animal 2
erythema	1 h	2	2
	24 h	2	2
	48 h	3	3
	8 d	2	2
edema	1 h	2	2
	24 h	2	4
	48 h	3	4
	8 d	2	2

4 hours exposure	time	animal 1	animal 2
erythema	4 h	3	3
	24 h	3	3
	4 d	4	3	#1: necrosis
	8 d	4	4	#1: scaling, #2: necrosis
edema	1 h	4	3
	24 h	4	4
	4 d	2	3
	8 d	2	3

Applicant's summary and conclusion

Interpretation of results:

Category 1C (corrosive) based on GHS criteria