Ethyldimethyl[2-[(2-methyl-1-oxoallyl)oxy]ethyl]ammonium ethyl sulphate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): MADAM MECL
- Physical state: Slightly viscous and colourless liquid.
- Composition of test material, percentage of components: 80% solution in water
- Lot/batch No.: no data
- Storage condition of test material: In darkness and at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

a stock solution (1,000 mgll) was prepared approximately 30 minutes before the start of the study, manually adding the test substance into dilution water,
• this stock solution was submitted to magnetic stirring during 10 minutes, • the stock solution was homogeneous and limpid,
•pH was not adjusted (6.86 at 22.3°C).

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

control group: 10 zebra fish
treated groups (3) : 30 zebra fish

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

90 mg/L CaCO3

Test temperature:

19.4-20.6

pH:

6.86-6.95

Dissolved oxygen:

97 mg/l

Salinity:

none

Nominal and measured concentrations:

Nominal, 0 (control), 1, 10 and 100 mg

Details on test conditions:

Water Quality:
• contaminants: no contaminant was present, (e.g. chlorine) at a concentration which could have prejudiced the study.
- dilution water as well as acclimatization water was reverse osmosed natural water (from 60 m depth) that was further added with appropriate salts to get the required characteristics;
- no food was provided during the study

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No abnormality was observed in the test media during the study
No abnormality of behaviour, pigmentation, morphology was observed
In the 10 and 100mg/L test groups fish formed a group and fish were excited during the first 24hours. Then return to normal.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the LC50 for MADQUAT MC at 96 hours for freshwater fish (Brachydanio rerio) was higher than 100 mg/L, the NOEC was 100 mg/L.