Isopropyl benzoate

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This is a summary of studies reviewed by the expert group of the U.S. Cosmetics Ingredient Review (CIR), which accepted the data as scientifically valid.

Data source

Reference

Reference Type:

review article or handbook

Title:

Safety assessment of alkyl benzoates as used in cosmetics

Author:

Becker LC, Bergfeld WF, Belsito DV, et al.

Year:

2012

Bibliographic source:

Int. J. Toxicol. 31:342S-72S

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 428 (Skin Absorption: In Vitro Method)

Principles of method if other than guideline:

Diffusion cells with excised guinea pig skin

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methyl benzoate

EC Number:

202-259-7

EC Name:

Methyl benzoate

Cas Number:

93-58-3

IUPAC Name:

methyl benzoate

Test animals

Species:

guinea pig

Administration / exposure

Details on study design:

The penetration of methyl benzoate, ethyl benzoate, n-propyl benzoate and n-butyl benzoate through excised guinea pig dorsal skin was measured using diffusion cells. Permealibility was increased by removal of the stratum corneum by tape stripping and delipidization using a chloroform-methanol mixture. Penetration was decreased by the addition of 1-methanol plus 15% ethanol.

Results and discussion

Signs and symptoms of toxicity:

not specified

Dermal irritation:

not specified

Percutaneous absorption

Remarks on result:

other:

Any other information on results incl. tables

Permeability coefficients:

	Permeability Coefficient Kp (x 10^-2cm/h)
Methyl benzoate	20.3 ± 5.8
Ethyl benzoate	34.1 ± 1.2
n-Propyl benzoate	62.7 ± 13.0
n-Butyl benzoate	79.9 ± 10.1

Permealibility was increased by removal of the stratum corneum by tape stripping and delipidisation using a chloroform-methanol mixture. Penetration was decreased by the addition of 1-methanol plus 15% ethanol.

Applicant's summary and conclusion

Conclusions:

In vitro dermal penetration studies of excised guinea pig skin demonstrates transport of methyl benzoate and related short-chain esters. This study is informative for evaluation of the toxicity and environmental fate of members of the Alkyl Benzoates category.