Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29 Oct - 29 Nov 2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other: ISO Standard 14669: Water Quality - Determination of Acute Lethal Toxicity to Marine Copepods (Copepoda, Crustacea), 1999.
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: Control and all concentrations
- Sampling method: Duplicate samples of at least 50 mL were taken
- Sample storage conditions before analysis: Stored at -18 to -25 °C prior to analysis
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 200 mg/L stock solution was prepared in synthetic seawater by stirring for 1 hour. The stock solution was used directly as the test medium. It is likely that the nominal concentration exceeded the solubility of the test substance in the test medium.
Test organisms (species):
other: Acartia tonsa (Dana)
Details on test organisms:
TEST ORGANISM
- Source: From laboratory stock culture at ECT Oekotoxicologie GmbH. The stock culture was originally supplied by the Technical University of Denmark, Lyngby, Denmark.
- Age at study initiation (mean and range, SD): 14-21 days
- Feeding during test: none

ACCLIMATION
- Type and amount of food: algae (Rhodomonas salina)
- Feeding frequency: daily
- Acclimation period in test medium: 9 days
Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
not reported
Test temperature:
19.7-21.1 °C
pH:
7.6-8.7
Dissolved oxygen:
7.8-8.5 mg/L
Salinity:
30.3 ppt
Nominal and measured concentrations:
Nominal concentrations: 0 (Control) and 200 mg/L
Measured concentrations were 79.3% of nominal at the start of the test and 72.5% of nominal at the end of the test
Measured initial: 0 and 151.9 mg/L

The measured concentration at the end of the test was 91.4% of the measured concentration at the start of the test.
Details on test conditions:
TEST SYSTEM
- Test vessel: beakers
- Type: covered by glass lids
- Material, size, headspace, fill volume: glass, 100 mL and containing between 50 and 80 mL of test medium
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic seawater prepared in accordance with Kusk and Wollenberger (1999). Fully defined saltwater medium for cultivation of and toxicity testing with marine copepod Acartia tonsa. Env Toxicol Chem 18:1564-1567
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: 539 Lux

EFFECT PARAMETERS MEASURED: Mortality after 24 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 151.9 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: 0%
Results with reference substance (positive control):
- Results with reference substance valid?: yes
- 48-h LC50: 0.749 mg/L (95% CI: 0.634-0.884 mg/L)
Reported statistics and error estimates:
No toxic effects were observed in the test and therefore statistical analysis of the results was not required.

Table 1. Results of analysis of test media 

Nominal test substance concentration (mg/L)

Mean measured concentration in fresh test medium at start of test (mg/L)

Mean measured concentration in old test medium after 48 hours (mg/L)

Mean measured concentration at start of test as percentage of nominal

Mean measured concentration after 48 hours as percentage of nominal

0 (Control)

0.8

1.1

-

-

192

152

139

79

73

 

No toxics effects were observed at the single test concentration of 151.9 mg/L (meas. init.) resulting into a NOEC (48 h) of >= 151.9 mg/L (meas. init.)

Validity criteria fulfilled:
yes
Conclusions:
A 48-h EC50 value of > 151.9 mg/L and a NOEC of ≥ 151.9 mg/L (both meas. init.) have been determined in the study (ISO 14669) testing the effects of 3-(triethoxysilyl)-N-[3-(triethoxysilyl)propyl]-1-propanamine towards Acartia tonsa.

Description of key information

EC50 (48 h) > 151.9 mg/L (meas. init., Acartia tonsa, ISO 14669)

Key value for chemical safety assessment

Marine water invertebrates

Marine water invertebrates
Dose descriptor:
EC50
Effect concentration:
> 151.9 mg/L

Additional information

Experimental data are available testing the acute toxicity of 3-(triethoxysilyl)-N-[3-(triethoxysilyl)propyl]-1-propanamine (CAS No. 13497-18-2) towards aquatic invertebrates. The study was performed according to the ISO guideline 14669 and GLP standards (2004). The salt water organism Acartia tonsa was used for testing and was exposed for 48 h in a static testing regime to one single nominal test item concentration of 200 mg/L and a control. The test item was monitored via measurement of DOC at the beginning and at the end of the experiment. The recoveries of the test substance were between 72.5 and 79.3%. No toxic effects were recorded up to the highest tested concentration and thus the study resulted into a 48-h EC50 value of > 151.9 mg/L (meas. initial).