Sodium hydrogen N-(1-oxotetradecyl)-L-glutamate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From January 26, 2016 to February 09, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

Batch no.: 3837
Purity: 25.5% in water
Solubility: soluble in water

Analytical monitoring:

yes

Remarks:

Measurement of analytical total organic carbon (TOC) concentrations with a TOC analyser

Details on sampling:

- Samples, properly diluted with deionised water with low TOC contents, have been analysed by means of an integrated sampling system directly from vials containing 40 ml volume. For each sample under test the corresponding blank is analysed.
- Samples were stable over the duration of the test (96 h).

Vehicle:

no

Details on test solutions:

Range-finding test: 5 different concentrations of the active ingredient (from 0.1 to 1000 mg/L).
Definitive test: solution at 100 mg/L of the active ingredient in water.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Length : 2 +/- 1 cm
Biomass-density: < 1 g/L
Number of animals: 7/tank
Supplier: Aquarium Center
Feeding: feedstuff in flakes

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

250 mg CaCO3/L

Test temperature:

23 +/- 2°C

pH:

7.5 - 8.1

Dissolved oxygen:

61 - 74%

Nominal and measured concentrations:

Range finding test: 5 different concentrations of the active ingredient (from 0.1 to 1000 mg/L).
Definitive test: solution at 100 mg/L of the active ingredient in water.

Details on test conditions:

Aeration: continuous with porous stones
(Tanks have been lighted with white light lamps with 12 h photoperiod daily).
NB: The water used has been allowed to stabilize for 48 h to greatly reduce chlorine presence.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

The test substance was considered stable, because the TOC values at the end of the test remain in the required range (80 -120%) respect to the initial values.

Results with reference substance (positive control):

-

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 96 h LC50 of the substance in Brachydanio rerio was determined to be >100 mg a.i./L.

Executive summary:

A study was conducted to determine the short-term toxicity of the substance to fish according to OECD Guideline 203, in compliance with GLP. Brachydanio rerio (7/group) were exposed to the test substance at a concentration of 100 mg/L for 96 h. The total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the test. No mortalities or symptoms (visible abnormalities) were observed during the test. Under the study conditions, the 96 h LC50 of the substance in Brachydanio rerio was determined to be >100 mg a.i./L (Giarei, 2016).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

100 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the substance to fish according to OECD Guideline 203, in compliance with GLP. Brachydanio rerio (7/group) were exposed to the test substance at a concentration of 100 mg/L for 96 h. The total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the test. No mortalities or symptoms (visible abnormalities) were observed during the test. Under the study conditions, the 96 h LC50 of the substance in Brachydanio rerio was determined to be >100 mg a.i./L (Giarei, 2016).