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EC number: 807-159-2 | CAS number: 69701-99-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Jan 2006 - 23 Feb 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Principles of method if other than guideline:
- “Method for Testing Bio-degradability of Chemical Substances by Microorganisms” stipulated in the “Testing Method for New Chemical Substances” (Yakushoku notification No 1121002 2003, Nov. 21, 2003, 11, 12, Seikyoku No. 2, Kanpokihatsu notification No. 0311221002)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Lot/batch number: 09891502
- Physical Appearance: colourless liquid
- Purity: 99.51% - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Storage conditions: 24.6 - 25.1 °C
- Storage length: 28 days
- pH: 6.4 - 8.6
- Concentration of sludge: 30mg/L
- Amount: 2.428mL
Preparation of Activated Sludge
Standard activated sludge used was purchased from Chemicals Evaluation and Research Institute, Japan.
Cultivation
About 1 L of activated sludge was aerated in the cultivation tank for 23.5 hours, About 30 min after ceasing aeration, supernatant corresponding to about 1/3 of the whole volume (approximately 330 mL) was removed. Then equal volume of 0.1 % synthetic sewage (1) was added to the remaining portion again. This procedure was repeated once a day.
(1) Synthetic sewage(0.1%) was prepared by mixing synthetic sewage (10%, 3.3 mL)) wth pure water (330 mL). Glucose, peptone, and potassium phosphate monobasic (20 g each) were dissolved in 200 mL of pure water and adjusted to pH 7.0 ± 1.0 with sodium hydroxide.
Parameters of cultivation
Temperature: 25 ± 2 °C
Dissolved Oxygen: > 5.0 mg/L
Sedimentation ratio of activated sludge: Flock was large, and Sedimentation ration of the activated sludge was high.
pH: 7.0 ± 1 (Supernatant solution)
Appearance of supernatant fluid: Clear
Concentration of supernatant solids: Depend on Condition of Activated Sludge, 0.1 % synthetic sewage is to be added if necessary.
Microorganism: Flock was thick, and was observed as aggregates. Protozoa (Ciliata) and Metazoan (Rotaria) were observed. - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Details on study design:
- TEST CONDITIONS
- Test temperature: 24.8 - 25.5 °C
- pH: 5.9 - 8.6
- pH adjusted: no
- Suspended solids concentration: 1000 mg/L
TEST SYSTEM
- Culturing apparatus: Cultivation tank
- Measuring equipment: Gas chromatograph, TOC meter, GC/MS system, coulomter, pH meter
SAMPLING
- Sampling frequency: 7 days
Preparation for Test
Each 6 mL of solution A, B, C and D, which are prescribed in the Japanese Industrial Standard (JIS) K0102-21, were made up to 2.0 L with pure water. The following solutions were prepared in the test bottles and adjusted at test temperature:
- 100 mg/L test substance in pure water: Bottle No 1.
- 100 mg/L test substance in basal culture medium: Bottle No 2, 3 and 4
- 100 mg/L aniline in basal culture medium: Bottle No. 5
- Basal culture medium for blank test: Bottle No. 6 - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 29.7
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 33.1
- Sampling time:
- 28 d
- Key result
- Parameter:
- other: GC
- Value:
- 100
- Sampling time:
- 28 d
- Details on results:
- MEDOL-10 was hydrolysed into acrylic acid and MEDOL-OH. Acrylic acid was a readily biodegradable substance dispersant from the test solution, while MEDOL-OH and MEDOL-COOH remained.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The mean percentage biodegradation of MEDOL-10 calculated by biological oxygen demand (BOD) dissolved organic carbon concentration (DOC) and gas chromatography (GC) was 29.7, 33.1 and 100 % respectively. The percentage biodegradation by BOD and DOC were similar, while that obtained by GC resulted in a comparatively high value. The percentages of the metabolites remaining were estimated.
The remaining metabolite was identified as MEDOL-COOH (4-carboxyl-2-ethyl-2-methyl-1,3-dioxolane). This metabolite was oxidised from MEDOL_OH (4-hydroxymethyl-2-ethyl-2-methyl-1,3-dioxolane), one of the two hydrates of test item, the other being acrylic acid. Acrylic acid is readily biodegradable substance and disappear from the test solution, while MEDOL-OH and MEDOL-COOH remained. - Executive summary:
Introduction
This biodegradability study of the test substance ( 4-Acryloyloxymethyl-2-ethyl
- 2-methyl-l, 3-dioxolane) was conducted in accordance with Ministerial ordinances requiring designation of the items, etc. of tests and the items of studies to be conducted in connection with a Type I Monitored Chemical Substance or Type II Monitored Chemical Substance related to a new chemical substance (2003.Nov.21, the order of the Minister of Health, Labour and Welfare, the Minister of Economy, Trade and Industry, and the Minister of the Environment No. 3, Paragraph 1, Article 2).Results
The mean percentage biodegradation of MEDOL-10 calculated by biological oxygen demand (BOD) dissolved organic carbon concentration (DOC) and gas chromatography (GC) was 29.7, 33.1 and 100 % respectively. The percentage biodegradation by BOD and DOC were similar, while that obtained by GC resulted in a comparatively high value. The percentages of the metabolites remaining were estimated.
The remaining metabolite was identified as MEDOL-COOH (4-carboxyl-2-ethyl-2-methyl-1,3-dioxolane). This metabolite was oxidised from MEDOL_OH (4-hydroxymethyl-2-ethyl-2-methyl-1,3-dioxolane), one of the two hydrates of test item, the other being acrylic acid. Acrylic acid is readily biodegradable substance and disappear from the test solution, while MEDOL-OH and MEDOL-COOH remained.
Conclusion
MEDOL-10 was hydrolysed into acrylic acid and MEDOL-OH. Acrylic acid was a readily biodegradable substance dispersant from the test solution, while MEDOL-OH and MEDOL-COOH remained.
Reference
Total amount of test substance and metabolites (MEDOL-OH and MEDOL-COOH)
| Substane Name | Item | Test Bottle No. | ||||
| No. 1 | No. 2 | No. 3 | No. 4 | No. 6 | ||
| MEDOL-10 | Production (mg) | 28.6 | 0 | 0 | 0 | 0 |
| (1) Production rate (%) | 95.3 | 0 | 0 | 0 | 0 | |
| MEDOL-OH | Production (mg) | 0.8 | 0.3 | 0.3 | 0.4 | 0 |
| (2) Production rate (%) | 2.7 | 1 | 1 | 1.3 | 0 | |
| MEDOL-COOH | Production (mg) | 0 | 18.1 | 16.5 | 17.1 | 0 |
| (3) Production rate (%) | 0 | 60.3 | 55 | 57 | 0 | |
| Total (1) + (2) + (3) | 98 | 61.3 | 56 | 58.3 | 0 | |
Percentage Biodegradation by BOD, DOC, GC and GC/MS analysis
| Item | Test Bottle No. | ||||
| No. 1 | No. 2 | No. 3 | No. 4 | No. 6 | |
| Percentage Biodegradation by BOC (%) | - | 29.2 | 28.6 | 31.4 | - |
| Percentage Biodegradation by DOC (%) | - | 33.2 | 33.3 | 32.8 | - |
| Percentage Biodegradation by residual amount of metabolites (%) | - | 38.7 | 44 | 41.7 | - |
| Percentage Biodegradation by residual amount of test substance (%) | - | 100 | 100 | 100 | - |
Description of key information
The mean percentage biodegradation of MEDOL-10 calculated by biological oxygen demand (BOD) dissolved organic carbon concentration (DOC) and gas chromatography (GC) was 29.7, 33.1 and 100 % respectively. The percentage biodegradation by BOD and DOC were similar, while that obtained by GC resulted in a comparatively high value. The percentages of the metabolites remaining were estimated.
The remaining metabolite was identified as MEDOL-COOH (4-carboxyl-2-ethyl-2-methyl-1,3-dioxolane). This metabolite was oxidised from MEDOL_OH (4-hydroxymethyl-2-ethyl-2-methyl-1,3-dioxolane), one of the two hydrates of test item, the other being acrylic acid. Acrylic acid is readily biodegradable substance and disappear from the test solution, while MEDOL-OH and MEDOL-COOH remained.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
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