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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Se!enastrum capricornutum
- Strain: Pseudokirchneriel/a subcapitata HINDAK, SAG 61.81
- Source (laboratory, culture collection): Collection of algal cultures in University of Göttingen /Germany
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Details on test conditions:
TEST SYSTEM
- Test vessel: Sterile Erlenmeyer flasks (volume 250 ml; sealed with cotton wool plugs).
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 24 hours/day light
- Light intensity and quality: Approximately 4440 to 8880 lux, corresponding to 60 to 120 μE*m-2*s-1


Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
>= 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
The study was conducted under static conditions with an initial cell density of 6839 cells/ml. A stock solution of 120 mg/L was freshly prepared with dilution water and agitated until the solution was visually clear and tested as a limit concentration. Six replicates were used for the limit concentration and the control. The environmental conditions were within the acceptable limits. The concentration of the test item Glycerol Monomethacrylate (GMMA) were analytically verified via LC-MS/MS at the start of the exposure (0 hours) and at the end of the exposure (72 hours) in the limit concentration and the control. The measured concentration of the test item at the start of the exposure (0 hours) was 94% of the nominal value. The measured concentrations at the end of the exposure (72 hours) was 97% of the nominal value. The geometric mean measured concentration was 112 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
In this study, no inhibition of growth on the freshwater green alga Pseudokirchneriella subcapitata was found after 72 hours exposure to the test item Glycerol Monomethacrylate (GMMA). Based on the nominal test item concentration the NOEC (0-72 hours) for both inhibition of growth rate and inhibition of yield was 120 mg/L. The EC50-values for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were > 120 mg/L, respectively. The geometric mean of the measured concentrations of the test item in the tested concentration was 112 mg/L.

Description of key information

In this study, no inhibition of growth on the freshwater green alga Pseudokirchneriella subcapitata was found after 72 hours exposure to the test item Glycerol Monomethacrylate (GMMA). Based on

the nominal test item concentration the NOEC (0-72 hours) for both inhibition of growth rate and inhibition of yield was 120 mg/L. The EC50-values for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were > 120 mg/L, respectively. The geometric mean of the measured concentrations of the test item in the tested concentration was 112 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
120 mg/L
EC10 or NOEC for freshwater algae:
120 mg/L

Additional information