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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 60, 140, 240 mg/L
- Sample storage conditions before analysis: Solutions were acidified with HNO3 for stabilisation, samples were analysed immediately after sampling or (if immeidate anaysis not possible) stored at 4°C.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared according to OECD guidance No. 23. The test solutions were prepared separately by adding the required amount of substance to the test medium. the solutions were stirred for 24 hours and subsequently filtered (0.2 µm filter).
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene. Specimens used in the test were bred in the laboratory of the Fraunhofer IME.
- Age at study initiation (mean and range, SD): 4-24 h
- Feeding during test: no


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
1.2 - 1.3 mmol/L
Test temperature:
19.6 - 19.7 °C
pH:
8.1 - 8.3
Dissolved oxygen:
7.8 - 8.9 mg/L
Nominal and measured concentrations:
Nominal test substance concentrations: 60, 120, and 240 mg/L (corresponding to 22.9, 45.7 and 91.4 mg Mn/L)
Measured Mn concentrations test start: 1.24, 2.02, 2.10 mg Mn/L (corresponding to 3.72, 5.31, 5.52 mg test substance/L)
Measured Mn concentrations test end: 0.74, 1.68, 2.18 mg mn/L (corresponding to 1.95, 4.41, 5.73 mg test substance/L)
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beakers (50 mL)
- Fill volume: 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water.
- Alkalinity: 2.0 - 2.4 mmol/L
- Conductivity: 227 - 224 µs/cm


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day-night regime
- Light intensity: 763 - 823 Lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 h

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 60, 120, 240 mg/L
- Results used to determine the conditions for the definitive study: EC50>240 mg/L
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 5.63 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Remarks:
recaluclated from measured Mn concentration
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 5.63 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Remarks:
Mn concentration
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 240 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 240 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: no
- Mortality of control: no
Reported statistics and error estimates:
No statistic test was performed since no daphnids died during the study.

Description of key information

Not acutely toxic to aquatic invertebrates.

Key value for chemical safety assessment

Additional information

The acute toxicity of trimanganese bis(orthophosphate) (CAS 14154-09-7) to aquatic invertebrates was investigated in a study conducted according to OECD guideline 202. Daphnia magna were exposed to nominal test substance concentrations of 60, 120, and 240 mg/L for 48 hours. The test concentrations were prepared separately by applying the Water Accommodated Fraction (WAF) approach according to the OECD guidance No. 23 and 29. The concentrations of trimanganese bis(orthophosphate) in the test media were determined by chemical analyses of manganese in the aqueous phase at the start and at the end of the test using ICP-OES (LOQ 9.78 μg Mn/L). The test substance concentrations were recalculated from the measured manganese concentrations. An immobilization of the Daphnids was not observed at any tested concentrations. After 48 hours of exposure an EC50(48 h) >5.63 mg/L (mean measured) was determined. Based on the nominal substance concentration an EC50(48h) > 204 mg/L was recorded (EC50(48 h).          

Based on the experimental results trimanganese bis(orthophosphate) is not acutely toxic to aquatic invertebrates.