2,6-Diamino-4,5,6,7-tetrahydrobenzothiazole

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 october 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted in GLP compliance and in accordance with several internationally established guidelines (OECD, EEC, EPA, see below).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

SND 919 Precursor (SND 855 BS)

IUPAC Name:

SND 919 Precursor (SND 855 BS)

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): SND 919 Precursor 2 (SND 855 BS)

- Name of test material (as cited in study report): SND 919 Precursor 2 (SND 855 BS); 2,6-Diamino-4,5,6,7-tetrahydrobenzothiazole
- Molecular formula (if other than submission substance): C7H11N3S
- Molecular weight (if other than submission substance): 169.25
- CAS no. 104617-49-4
- Physical state: Solid, powder, grey
- Analytical purity: 98 %
- Purity test date: 17 october 2003
- Lot/batch No.: 14
- Expiration date of the lot/batch: October 2005
- Storage condition of test material: At room temperature, dark and dry

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test medium of the test concentrations were prepared by dilution of a stock solution. Stock solution: 80 mg test item was diluted in 500 ml test water. Adequate volumes of this test medium were diluted with test water to prepare the test media. The test media were prepared just before introduction of test Daphnia (=start of the test).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Source: National Institute of Public Health, Hungary
- Age at study initiation (mean and range, SD): 7.7 - 22.6 hours old
- Sex: female
- Breeding Conditions: The Daphnia were bred in the Laboratories of LAP International Hungary under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test.


ACCLIMATION
- Acclimation period: for 7.7 hours under test conditions
- Acclimation conditions (same as test or not): yes, same
- Feeding frequency: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Water hardness was reported as 164.2 mg/L CaCO3

Test temperature:

18 to 22°C +- 1°C

pH:

from 7.28 to 8.91

Dissolved oxygen:

The dissolved oxygen concentration in the test media was always >60% of the air saturation value at the temperature used

Salinity:

not applicable

Nominal and measured concentrations:

nominal concentrations 5.0, 10.0, 20.0, 40.0 and 80.0 mg test item /L, and a control
measured concentration 5.47, 10.92, 22.05, 43.83, 87.56 mg/L

Details on test conditions:

TEST SYSTEM
The Test vessels were glass beakers of 40 ml test medium. Each concentration had 4 replicate vessels and each vessel contain 5 test organisms. The age of the test organisms were < 24 hours and they were not fed during the test. Vessels were kept at 18 to 22°C .

The dilution water was synthetic freshwater with a hardness of 164.2 mg/L CaCO3.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 19.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: .

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 25.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: .

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Table 1 Influence of test item on the mobility of Daphnia magna

Concentration (nominal) [mg/L]	No. of Daphnia tested	No. of immobilized Daphnia after		% of immobilized Dapnia after
		24 h	48 h	24 h	48 h
Control	20	0	0	0	0
5.0	20	0	0	0	0
10.0	20	4	5	20	25
20	20	6	9	30	45
40.0	20	13	17	65	85
80	20	20	20	100	100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 24 hour and 48 hour EC50 and their 95 % confidence limits were 25.2 mg/L (19.8-31.9 mg/L) and 19.2 mg/L (15.4-24.0 mg/L), respectively. The 48 hour NOEC (highest test concentration tested without toxic effects after the exposure period of 48 hours), respectively the 48 hour EC0 of SND 919 Precursor 2 (SND 855 BS) to Daphnia magna was determined to be 5.0 mg test item /L. The 48 hour EC100 was 80.0 mg/L test item /L.