2-chlorophenyl cyclopentyl ketone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003-09-08 to 2003-12-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorophenyl cyclopentyl ketone

EC Number:

229-802-0

EC Name:

2-chlorophenyl cyclopentyl ketone

Cas Number:

6740-85-8

Molecular formula:

C12H13ClO

IUPAC Name:

2-chlorophenyl cyclopentyl ketone

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study reports): JNJ-54582034-AAA (T003642)
- Physical state: liquid
- Appearance: yellow liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): non-adapted sludge from the Marl-Ost municipal sewage treatment plant
- Initial cell/biomass concentration: The number of bacteria in the inoculum was determined by the Koch pour-plate method. The inoculum had a bacterial count of 110 * 104 CFU/ml (colony forming units/ml).

Duration of test (contact time):

29 d

Initial test substance concentration

Initial conc.:

ca. 21.9 - ca. 22.1 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Solubilising agent (type and concentration if used):
- Test temperature: 20.1 - 22.?°C (mean: 21.8°C).
- pH: 7.5-7.6

TEST SYSTEM
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: Degradation was followed over the total test duration of 29 days after 0, 4, 8, 11, 14, 18, 22, 28 and 29 days via TIC analysis of the bound CO2

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (CO2 evolution)

Value:

-8

Sampling time:

28 d

Any other information on results incl. tables

The control item sodium benzoate achieved a degree of degradation of 98% in the course of 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

The test item studied reached a degree of degradation of -8% in the course of 28 days (mean of the test item batches). The negative value of the degree of degradation is due to mathematical calculations.