(3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

(3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride

EC Number:

255-578-9

EC Name:

(3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride

Cas Number:

41892-01-7

Molecular formula:

C17H37ClNO.Cl

IUPAC Name:

(3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Weight of animals: Male 127 - 147g; Female 127 - 141g, Age of animals: Male 57 - 62 days; Female 57 - 58 days. Acclimation period before start of test: 5 days. Animals per cage: 1. Room temperature: 21°C +/- 2 °C. Relative humidity: 50-60%. Hygiene: Laboratory and cage cleaning using commercially available antispticka, instrument and device disinfection by autoclave and hot-air sterilizer.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

test solution: pH 7.1, density 1007mg/ml. Dilution factors of test solution 1.47 and 2.15. The corresponding concentrations were 685 mg / kg and 468 mg / kg, assuming an initial concentration. Of 1007 mg / ml.

Doses:

Test solution

No. of animals per sex per dose:

Animals per dose: 5

Details on study design:

Duration of observation period following administration: 14 days.
Dose; 2.15 - 4.64 ml/kg. Animals per dose: 5

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

2 372 mg/kg bw

Effect levels 2

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

3 066 mg/kg bw

Mortality:

First clinical symptoms occurred 20 minutes after substance delivery and lasted with the surviving maximum up to 1 week. Lethality prevailed over the first two days post Application and further deaths occurred in the following 3 days.

Clinical signs:

other: The poisoning pattern was predominantly characterized by symptoms such as trembling, stilt, sluggish gait, salivary flow, ruffled fur, diarrhea and inflated or bloated belly, indicating a disturbance of the vegetative nervous system. In addition, the male

Any other information on results incl. tables

Dose (mg/kg)	Sex	Mortality (x/n)	Mortality (%)
1473	M	0/5	0
2165	M	1/5	20
3182	M	2/5	40
4672	M	5/5	100
1007	F	0/5	0
2165	F	2/5	40
4672	F	5/5	100

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

LD50 of QUAB 342 test sample (28% aqueous solution) was determined as 2372 mg/kg bw for female animals and 3066 mg/kg bw for male animals.

Based on the lower value of 2372mg/kg bw, this is equiivalent to an LD50 of 664 mg/kg bw for (3-chloro-2-hydroxypropyl)dodecyldimethylammonium chloride.