Reaction products of diazotised 3-amino-2-hydroxy-5-nitrobenzenesulphonic acid, coupled with 1,3-diaminobenzene and diazotised sodium 4-aminobenzenesulfonate, metallised with Basic Chromium (III) Sulphate

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 (48 h, Daphnia magna) = 213.60 mg/L (extrapolated, based on nominal conc. verified by measurments)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

213.6 mg/L

Additional information

A static test was performed according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation (EC) No. 440/2008. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

Three concentrations showed significant toxicity between 10 and 40 % immobilisation. None of the animals was immobilised in the blank control.

At the beginning and at the end of the test, the content of the test item in the test solutions was determined usinga photometer. The concentrations determined at the start of the test were between 95 % and 97 % of the nominal concentrations. At the end of the test the determined concentrations were between 110 % and 116 % of the nominal concentrations. Because of evaporation, the concentrations slightly increased during the test. The determination of the biological results was based on the nominal concentrations.

The positive control tested in a current reference study, assured that the test conditions were reliable.

No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met. The result of the test is considered valid.