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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
31 Jul 1980 to 14 Aug 1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Version / remarks:
in accordance with Japanese guidelines (Kapogyo No. 5, Yakuhatsu No. 615 nad 49 Kikyoku No. 392)
Principles of method if other than guideline:
The oxygen demand of the test substance was determined after 14 days incubation. The study is conducted as a preliminary study.
GLP compliance:
no
Specific details on test material used for the study:
- Name of the test substance (as cited in study report): PGA-X
Oxygen conditions:
aerobic
Inoculum or test system:
other: sludge (not further specified)
Details on inoculum:
- Source of sludge: Received from Chemical Biotesting Center on May 13, 1980.
Duration of test (contact time):
14 d
Initial conc.:
30 mg/L
Based on:
test mat.
Remarks:
corresponds to 62.6 mg ThOD (The ThOD value was corrected to 62.6 mg by the assessor. Orginial value was 73.3 mg ThOD)
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
Biological oxygen demand (BOD)
Details on study design:
TEST SYSTEM
- Temperature: 25 °C
- Sludge concentration: 100 mg/L

MEASUREMENTS: O2 demand
After 14 days, the biological oxygen demand was determined using a Automatic oxygen determination system.
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
1.8
Sampling time:
14 d
Remarks on result:
other: Biodegradation value was corrected to 1.8% by the assessor. Orginial biodegradation value was 1.4% (calculated based on an incorrect ThOD-value)
Details on results:
The BOD of the test substance was determined to be 1.1 mg after 14 days.
Results with reference substance:
The degree of biodegradation of the reference substance after 7 days was 70.7 %.
Validity criteria fulfilled:
not specified
Interpretation of results:
not readily biodegradable
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
Supplementary estimation of ready biodegradability using BIOWIN v4.10
Principles of method if other than guideline:
BIOWIN v4.10 prediction for readily biodegradation.
GLP compliance:
no
Specific details on test material used for the study:
- SMILES: C3OC3CN(CC4CO4)Cc1cccc(CN(CC5CO5)CC2CO2)c1
Parameter:
probability of ready biodegradability (QSAR/QSPR)
Remarks:
Biowin2 (Non-Linear Biodeg Probability)
Value:
0
Remarks on result:
not readily biodegradable based on QSAR/QSPR prediction
Remarks:
Substance does not fall within the applicability domain of the model.
Parameter:
probability of ready biodegradability (QSAR/QSPR)
Remarks:
Biowin6 (MITI Non-Linear Biodeg Probability)
Value:
0.001
Remarks on result:
not readily biodegradable based on QSAR/QSPR prediction
Remarks:
Substance does not fall within the applicability domain of the model.
Details on results:
A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable
A Probability Less Than 0.5 indicates --> NOT Readily Degradable

Endpoint

Result

Biowin1 (Linear Model Prediction)

Does Not Biodegrade Fast

Biowin2 (Non-Linear Model Prediction)

Does Not Biodegrade Fast

Biowin3 (Ultimate Biodegradation Timeframe)

Months

Biowin4 (Primary Biodegradation Timeframe)

Weeks-Months

Biowin5 (MITI Linear Model Prediction)

Not Readily Degradable

Biowin6 (MITI Non-Linear Model Prediction)

Not Readily Degradable

Biowin7 (Anaerobic Model Prediction)

Does Not Biodegrade Fast

Ready Biodegradability Prediction

NO

The test substance does not fall within the applicability domain of the model as the number of some fragments exceeds the maximum number of fragments identified within the training set (Appendix D of the On-Line BIOWIN(TM) User's Guide).

Please refer to the full results report attached to this IUCLID record.

Interpretation of results:
not readily biodegradable

Description of key information

The test substance was determined to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The biodegradation potential of the substance in water was determined in a non-GLP preliminary screening study similar to OECD TG 301C (Modified MITI Test) (Niigata Research Laboratory, 1980). In this study, 30 mg/L test substance was inoculated with sludge for 14 days under aerobic conditions. During the incubation period the biological oxygen demand (BOD) was measured and thereafter compared to the theoretical uptake (ThOD), to determine the degree of biodegradation. After the 14-day incubation period 1.8% of the substance was biodegraded. Although the test substance was not incubated for the prescribed 28 days, a worst-case conclusion is drawn based on 14 days incubation that the substance is not readily biodegradable.

To support this outcome, BIOWIN v4.10 data was generated on the test substance as part of a weight-of-evidence approach. The QSAR model predicts that the substance is not readily biodegradable. Even though the substance does not (completely) fall within the applicable domain, the results can still be used to support the study results. Therefore, the substance is considered not readily biodegradable.