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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
weight of evidence
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Please refer to attached docum
Principles of method if other than guideline:
Assessment of inhibition of cell multiplication by the protozoon, Uronema parduzci. The number of protozoa was determined by means of a cell counter.
GLP compliance:
not specified
Analytical monitoring:
not specified
not specified
Test organisms (species):
Uronema parduzci
Test type:
Water media type:
Limit test:
Total exposure duration:
20 h
Test temperature:
Details on test conditions:
Number of protozoa by means of a cell counter
The protozoa cultures are fed with bacterial suspensions of Escherichia coli

For mathematical evalutation of toxicological findings at the end of the test period, the mean value (A) of impulses/ml in test cultures free from toxic
influence or non-stimulated test cultures is calculated, provided the counts are within a standard deviation range of <5%. The mean value (B) of counts performed in test cultures of the lowest toxic pollutant concentration of the dilution series are also calculated.
As coordinates, (a) (highest non-toxic pollutant concentration) over (A) and (b) (lowest toxic pollutant concentration) over (B) are entered into a suitable electronic calculator. After having entered (A - 5%), the pollutant concentration at which inhibitory action begins (c) may be obtained from the regression line between (a;A) to (b;B). The findings obtained are given in the following paragraphs for pollutant concentrations at which the onset of inhibitory action was observed have been obtained by mathematical evaluation of counts.
Key result
20 h
Dose descriptor:
other: TGK
Effect conc.:
22 mg/L
Basis for effect:
growth inhibition
Validity criteria fulfilled:
not specified
Diallyl phthalate is not reported as toxic to the protozoan Uronema parduzci with a threshold concentration of 22 mg/l.

Description of key information

DAIP read-across from DAP - The key value is based on published studies with four different species: Pseudomonas putida, Entosiphon sulcatum, Uronema parduzci and Chilomonas paramecium. Although the separate studies themselves are not sufficient enough to assess a key value for the toxicity of diallyl phthalate in micro-organisms, the combination of these four publications give a reliable value.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
22 mg/L

Additional information

Diallyl phthalate, a closely related structural analogue for DAIP, proved to be non-toxic for micro-organisms based on the results of various studies with four different species.