Diallyl isophthalate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

ANALOGUE APPROACH JUSTIFICATION
Please refer to attached docum

Principles of method if other than guideline:

Assessment of inhibition of cell multiplication by the protozoon, Uronema parduzci. The number of protozoa was determined by means of a cell counter.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Uronema parduzci

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

20 h

Test temperature:

25°C

Details on test conditions:

EFFECT PARAMETERS MEASURED
Number of protozoa by means of a cell counter
The protozoa cultures are fed with bacterial suspensions of Escherichia coli

EVALUATION
For mathematical evalutation of toxicological findings at the end of the test period, the mean value (A) of impulses/ml in test cultures free from toxic
influence or non-stimulated test cultures is calculated, provided the counts are within a standard deviation range of <5%. The mean value (B) of counts performed in test cultures of the lowest toxic pollutant concentration of the dilution series are also calculated.
As coordinates, (a) (highest non-toxic pollutant concentration) over (A) and (b) (lowest toxic pollutant concentration) over (B) are entered into a suitable electronic calculator. After having entered (A - 5%), the pollutant concentration at which inhibitory action begins (c) may be obtained from the regression line between (a;A) to (b;B). The findings obtained are given in the following paragraphs for pollutant concentrations at which the onset of inhibitory action was observed have been obtained by mathematical evaluation of counts.

Key result

Duration:

20 h

Dose descriptor:

other: TGK

Effect conc.:

22 mg/L

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not specified

Conclusions:

Diallyl phthalate is not reported as toxic to the protozoan Uronema parduzci with a threshold concentration of 22 mg/l.

Description of key information

DAIP read-across from DAP - The key value is based on published studies with four different species: Pseudomonas putida, Entosiphon sulcatum, Uronema parduzci and Chilomonas paramecium. Although the separate studies themselves are not sufficient enough to assess a key value for the toxicity of diallyl phthalate in micro-organisms, the combination of these four publications give a reliable value.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

22 mg/L

Additional information

Diallyl phthalate, a closely related structural analogue for DAIP, proved to be non-toxic for micro-organisms based on the results of various studies with four different species.