Diallyl isophthalate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 to 12 May 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0.1, 0.18, 0.32, 0.53 and 1.0 mg/ l
- Sampling method: The test solution for analysis were sampled at two renewal sets of four during 96-hour exposure.
- Sample storage conditions before analysis:

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Test solutions were prepared by diluting WSF at a loading rate of 100 mg/l, concentrations are expressed in values calculated from the dilution rates.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Medaka
- Age at study initiation (mean and range, SD): < 1 year
- Source: Kitamura-yourijou (388 Gunchiku 12-bancho, Yatsushiro City, Kumamoto, Japan
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 2.36 cm
- Weight at study initiation (mean and range, SD): 0.105 g
- Feeding during test: no
- Food type: TetraMin, TetraWerke


ACCLIMATION
- Acclimation period: 11 days
- Acclimation conditions: water; Dechlorinated water, procedure; flow-through (biomass loading: 1 g fish/l/day or less), temperature, 24 ± 1°C, dissolved oxygen, 80% or more of the saturation; pH, 6.0 - 8.5; light, fluorescent light, 16 h light (1000 lux or less) / 8 hour dark; medication, not applied.
- Type and amount of food: TetraMin, TetraWerke; 2% of the bodyweight / day (approx.)
- Feeding frequency: no data
- Health during acclimation: Mortality during 7 days before the test, less than 5%

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

10-250 mg CaCO3 / l

Test temperature:

24 ± 1°C

pH:

6.7 - 8.5

Dissolved oxygen:

no data

Salinity:

Sodium 8.7 mg / l

Nominal and measured concentrations:

Nominal 0.10, 0.18, 0.32, 0.56 and 1.0 mg/l
measured 0.0149, 0.0272, 0.0501, 0.0861, 0.161

Details on test conditions:

TEST SYSTEM
- Test vessel: 5l glass vessel (cover: cleat PVC plate)
- Material, size, headspace, fill volume:
- Aeration: not aerated
- Renewal rate of test solution: every 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 g fish/l or less

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated water
- Total organic carbon: < 0.3 mg/l
- Particulate matter: < 1.0 mg/l
- Metals: < 0.02 mg/l
- Pesticides: < 0.002 mg/l
- Chlorine: < 0.1 mg / l
- Alkalinity: 33 mg CaCO3 / l
- Ca/mg ratio: 3.68
- Conductivity: 14 mS / m

OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark
- Light intensity: 1000 lux or less


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Test concentrations: 0.1, 1.0, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.118 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL 0.0861 - 0.161 mg/l

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.086 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

0.161 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no data
- Observations on body length and weight: l = 2.36 cm (2.15 to 2.66 cm); wt = 0.105 g (0.079 to 0.133 g). These values were taken from the control fish at the end of exposure.
- Other biological observations:
- Mortality of control: one fish died after 96h in the control
- Other adverse effects control: no data
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no data

Validity criteria fulfilled:

yes

Conclusions:

The 98 hour LC50 was found to be 0.118 mg/l

Description of key information

Acute Fish Toxicity Study 

The 96hr LC50 end point for DAP has been determined to be 0.23 mg/L. The 96h LC50 for DAIP has been determined to be 0.118 mg/L. However, this does not change the classification. DAP and DAIP are Aquatic Acute 1 as they both have 96h LC50 <= 1 mg/l.

 

The chemistry of the substances has already been shown to be similar. Although DAIP has a slightly lower water solubility than DAP the difference is unlikely to affect the end point of the study. Please refer to read-across justification.

 

Justification for the use of read-across. 

The physicochemical results demonstrate that the two substances are very similar. DAP and DAIP are both fairly soluble and have very similar log Pow values; DAP, 3.23 and DIAP, 3.36. These are important results as the Acute Fish Study is a crucial marker in determining the use of read-across for the aquatic compartment.  

It is on this basis that it is proposed that DAP is a suitable read-across substance for DAIP for aquatic endpoints.  

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.118 mg/L