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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Version / remarks:
adopted July 17, 1992
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Identification: Quaternium-26
Appearance: Clear amber to dark amber liquid
Batch: 0001954899
Purity/Composition: UVCB
Test item storage: At room temperature
Stable under storage conditions until 22 December 2017 (retest date)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
The freshly obtained sludge was used immediately. The concentration of suspended solids was determined to be 3.57 g/L in the concentrated sludge.
For experiment A, the sludge was allowed to settle (47 minutes) and the supernatant liquid was used as inoculum at the amount of 10 mL/L of mineral medium.
For experiment B, the sludge was allowed to settle (45 minutes) after which the supernatant was funnelled off and the sludge was made up to the initial volume using ISO-medium. The sludge was aerated (182 minutes) and diluted with ISO-medium to reach a SS concentration of 3.0 g/L. Magnetically stirred sludge was used as inoculum at the amount of 10 mL/L of mineral medium. The SS concentration in the test bottles of experiment B was 30 mg/L.
Duration of test (contact time):
28 d
Initial conc.:
25 mg/L
Based on:
test mat.
Remarks:
The test item was tested in duplicate at a target concentration of 25 mg/L, corresponding to 12 mg TOC/L.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
Test duration:
- 28 days for the inoculum blank and test item (last CO2measurement on day 29).
- 14 days for the positive and toxicity control (last CO2 measurement on day 15).
Test vessels: 2 litre brown coloured glass bottles.
Pre-incubation medium: The day before the start of the test (day -1) mineral components, Milli-RO water (ca. 80% of final volume) and inoculum (1% of final volume) were added to each bottle. This mixture was aerated with synthetic CO2-free air overnight to purge the system of CO2.
On the day of testing the test item was added to the 2-litres test bottles containing medium with microbial organisms and mineral components, using weighed amounts on a small watch glass. For experiment A, these amounts were: test item bottle A: 49.8 mg; test item bottle B:50.0 mg. For experiment B, these amounts were: test item bottle A: 50.2 mg; test item bottle B: 49.9 mg.
Additional bottles: 2 bottles for blank with inoculum; 2 bottles for the propylene glycol control (solvent control), 1 bottle for the Positive control: containing reference item and inoculum or the toxicity control containing reference item with test item and inoculum
Preparation: At the start of the test (day 0), test and reference item were added to the bottles containing the microbial organisms and mineral components. The volumes of suspensions were made up to 2 litres with Milli-RO water, resulting in the mineral medium described before. Three CO2-absorbers (bottles filled with 100 mL 0.0125 M Ba(OH)2) were connected in series to the exit airline of each test bottle.
Determination of CO2:
The CO2 produced in each test bottle reacted with the barium hydroxide in the gas scrubbing bottle and precipitated out as barium carbonate. The amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 M standardized HCl (1:20 dilution from 1 M HCl (Titrisol® ampoule), Merck, Darmstadt, Germany). Test item titrations were made on day 1, 4, 6, 8, 11, 15, 18, 22, 25 and 29.
On the penultimate day, the pH of respective test suspensions was measured and 1 mL of concentrated HCl (37%, Merck) was added to the bottles of the inoculum blank and test suspension. The bottles were aerated overnight to drive off CO2 present in the test suspension.
The final titration was made on day 15 for positive and toxicity control, and on day 29 for the remaining vessels.
Reference substance:
acetic acid, sodium salt
Key result
Parameter:
% degradation (CO2 evolution)
Remarks:
Based on measurements of two separate bottles.
Value:
122
St. dev.:
5
Sampling time:
28 d
Details on results:
The relative biodegradation values calculated from the measurements performed during the test period revealed 102% and 108% biodegradation of Quaternium-26, for A and B, respectively (based on ThCO2). Furthermore, biodegradation of Quaternium-26 of at least 60% was reached within a 10-day window.
The relative biodegradation values calculated for the solvent control revealed 77% and 79% biodegradation for A and B, respectively.
The relative biodegradation values calculated from the measurements performed during the test period revealed 117% and 127% biodegradation of Quaternium-26 (corrected for PG), for A and B, respectively (based on ThCO2).
Results with reference substance:
> 80% within 15 days.
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Remarks:
k1st order = 0.0173x, R² = 0.9703
Conclusions:
In conclusion, Quaternium-26 was readily biodegradable under the conditions of the modified Sturm test presently performed in experiment B. According to the OECD 301 Guideline, showing ready biodegradability in a single test is sufficient to classify a test item as readily biodegradable. Therefore, it can be concluded that Quaternium-26 is readily biodegradable.

Description of key information

Quaternium-26 was readily biodegradable under the conditions of a modified Sturm test. According to the OECD 301 Guideline, showing ready biodegradability in a single test is sufficient to classify a test item as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information