1,3,3-trimethyl-2-[2-(1-methyl-2-phenyl-1H-indol-3-yl)vinyl]-3H-indolium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride

EC Number:

228-800-7

EC Name:

2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride

Cas Number:

6359-50-8

Molecular formula:

C22H25N2.Cl

IUPAC Name:

1,3,3-trimethyl-2-[2-(2-methyl-2,3-dihydro-1H-indol-1-yl)vinyl]-3H-indolium chloride

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Preparation with stock solution of 10 mg/L and dilution water

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Strain optained from BGA, Berlin

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.1 - 21.0°C

Dissolved oxygen:

8.2 - 8.7 mg/L

Nominal and measured concentrations:

Nominal: 0 (control>), 0.091, 0.16, 0.53, 0.95 and 1.7 mg/L

Details on test conditions:

Water quality parameters according to guideline
Dilution water / test medium: Elendt M4
Replicates (test concentration, negative and positive controls): 3
Loading: according to guideline

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.29 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: geometric mean EC0/EC100

Results with reference substance (positive control):

- Dose-response test: concentrations 0.5, 1.0, 1.5, 2.0, 3.0, 4.0 and 5.0 mg/L
- EC50 (24h) 2.4 mg/L (Probit analysis)

Any other information on results incl. tables

concentration [mg/L]	cumulative immobilisation rate [%]
	24h	48h
control	0	0
0.09	0	0
0.16	0	0
0.29	45	95
0.53	75	100
0.95	90	100
1.7	100	100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h- EC50 to Daphnia magna was calculated to be 0.22 mg/L. The substance is acutely toxic to aquatic invertebrates.