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Registration Dossier
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EC number: 225-097-9 | CAS number: 4657-00-5
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 08 Jan - 05 Feb 1990
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
- EC Number:
- 228-800-7
- EC Name:
- 2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
- Cas Number:
- 6359-50-8
- Molecular formula:
- C22H25N2.Cl
- IUPAC Name:
- 1,3,3-trimethyl-2-[2-(2-methyl-2,3-dihydro-1H-indol-1-yl)vinyl]-3H-indolium chloride
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Concentration: 1 g/L dry matter
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- other: discolouration
- Details on study design:
- Blank control performed
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 27
- Sampling time:
- 28 d
- Parameter:
- other: % degradation (based on discolouration)
- Value:
- 53
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / COD
- Parameter:
- COD
- Value:
- 1 880 mg O2/g test mat.
- Remarks on result:
- other: 1 g/L
- Remarks:
- Method: DIN H41
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The substance showed a maximum biodegradation rate of 27% (based on DOC removal). The substance is not considered to be inherently biodegradable. Discolouration is not considered a suitable method to determine biodegradation, especially since the yellowish colouration of the test medium cannot be clearly related to the test item when activated sludge is added.
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