2,6-di-tert-butyl-4-nonylphenol

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Test material information

Constituent 1

Reference substance name:

2,6-di-tert-butyl-4-nonylphenol

EC Number:

224-320-7

EC Name:

2,6-di-tert-butyl-4-nonylphenol

Cas Number:

4306-88-1

Molecular formula:

C23H40O

IUPAC Name:

2,6-di-tert-butyl-4-nonylphenol

Test material form:

liquid

Details on test material:

- Density: 0.895 g/cm3

Radiolabelling:

no

Study design

HPLC method

Details on study design: HPLC method:

The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix was used (EC 50/4.6 Nucleodur 100-5 CN-RP 150 x 4.6 mm; 5 µm). A mixture of 55% methanol / 45 % ultra-pure grade water was used as mobile phase and the column temperature was 25°C ± 1°C.

Results and discussion

Adsorption coefficient

Key result

Sample No.:

#1

Type:

log Koc

Value:

6.93

Temp.:

25 °C

Results: HPLC method

Details on results (HPLC method):

The study reports the following values: 6.55-7.20 of which the average is 6.93 (key value). The range of 6.55-7.20 is above the determination limit of the method (log KOC 5) and above the highest reference standard 5.63). The above values were hence extrapolated.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The study reports the following values: 6.55-7.20 of which the average is 6.93 (key value).

Executive summary:

The adsorption coefficient of the test item on solid and on sewage sludge (K_OC) was determined by HPLC according to EC method C.19 (440/2008) and OECD guideline 121, under GLP. The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix was used (EC 50/4.6 Nucleodur 100-5 CN-RP 150 x 4.6 mm; 5µm). A mixture of 55% methanol / 45 % ultra-pure grade water was used as mobile phase and the column temperature was 25°C±1°C. The study reports the following values: 6.55-7.20 of which the average is 6.93 (key value). The range of 6.55-7.20 is above the determination limit of the method (log K_OC5) and above the highest reference standard 5.63). The above values were hence extrapolated. The determined log K_OCof two runs is within a range of±0.25 log units (absolute deviation). Hence the requirements of OECD guideline 121 concerning the quality criteria are fulfilled.