Cuprate(3-), [3-hydroxy-4-[[2-hydroxy-5-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-2,7-naphthalenedisulfonato(5-)]-, potassium sodium

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1993-04-05 to 1993-10-11

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

modified to differentiate between toxic effects and indirect effects by light absorption

Deviations:

yes

Remarks:

due to the modification for dyestuffs

Principles of method if other than guideline:

- Principle of test: two part of the test:
part A: the algae grew in test media with dissolved dyestuff in Erlenmeyer flasks, each placed in a black cylinder. The cylinders were covered with glass dishes, containing untreated test water.
part B: the algae grew in test media without dissolved dyestuff in Erlenmeyer flasks, each placed in a black cylinder. The cylinders were covered with glass dishes, containing coloured dyestuff solutions with the same five test concentrations as in part A (without algae).
- Parameters analyzed / observed: part A: toxic effect and indirect effect by light absorption; part B: indirect effect by light absorption

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

EC Number:

263-856-6

EC Name:

Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

Cas Number:

63105-49-7

Molecular formula:

C20H14CuN3O15S4.3Na C20H14CuN3Na3O15S4

IUPAC Name:

trisodium 15-acetamido-7-[2-(sulfonatooxy)ethanesulfonyl]-10λ³,12λ³-dioxa-2λ⁴,3-diaza-11-cuprapentacyclo[11.8.0.0²,¹¹.0⁴,⁹.0¹⁴,¹⁹]henicosa-1(21),2,4,6,8,13,15,17,19-nonaene-11,11-bis(ylium)-10,12-diide-17,21-disulfonate

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Nominal and measured concentrations:

Nominal: 0 (control), 1.0, 3.2, 10.0, 32.0 and 100 mg/L

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

10.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Experimental part A (toxic and indirect effects)

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

19.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Experimental part B (indirect effects)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

36.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Experimental part A (toxic and indirect effects)

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

61.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other:

Remarks:

Experimental part B (indirect effects)

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Based on toxic and indirect effects due to light absorption, the 72-h ErC50 to Scenedesmus subspicatus was determined to be 36.1 mg/L. The 72-h ErC50 considering real algicidal effects is 61.4 mg/L. The 72-h ErC10 was not determined exactly but was reported as >= 10 mg/L in the ETAD summary. The test item is considered acutely harmful to to freshwater green algae. The test item is the hydrolyzed form of the reactive dye. Since reactive dyes moderately hydrolyse in water at neutral pH conditions, this is considered the environmentally relevant form of the test item.