2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

EC Number:

303-085-5

EC Name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Cas Number:

94158-14-2

Molecular formula:

C9H11NO3.ClH

IUPAC Name:

2-(1,3-benzodioxol-5-ylamino)ethanol hydrochloride

Test material form:

solid: crystalline

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

17.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

ln this study AMINOL (A098) was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at nominal concentrations <: 25 mg/L (biomass growth and growth rate). The EC50-values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were 17.9 (15.2- 21.2) and 56.0 (47.7- 65.7) mg/L, respectively. Inhibition effects were found to be reversible up to the nominal concentration of 100 mg/L (all effect levels given as nominal concentrations).

Executive summary:

The toxicity of AMINOL (A098) to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201 and the EC Directive 92/69/EC, C.3., at DR.U.NOACK-LABORATORIUM FüR ANGEWANDTE BIOLOGIE in 31157 Sarstedt, Germany from July 8 to 11, 2003. The aim of the study was to assess the NOEC, LOEC and EC50 values of

growth rate and biomass production over a period of 72 h. AMINOL (batch R0075037) contained 99.6 % (w/w) 2-(1 ,3-benzodioxole-5-ylamino)ethanolhydrochloride. The study was conducted under static conditions with an initial cell density of nominally 104 cells/ml. On the basis of a preliminary test, live concentrations of nominal 6.25- 12.5- 25- 50- 100 mg/L were tested in a geometrical series with a dilution factor of 2. Three replicates were tested for each concentration level and six replicates for the control. Microscopic evaluation of the cells at the start and end of the incubation period revealed no morphological abnormalities. Water quality parameters like pHvalue, measured at 0 and 72 h, and room temperature, measured continuously, were determined to be within the acceptable limits. The concentrations of 2-(1 ,3-benzodioxole-5 - ylamino)ethanolhydrochloride were analysed at all concentration levels at test start and test end via HPLC. The mean values of recovery rates of the active ingredient at the beginning of the test were in the range of 91 to 100 %. At test end concentrations of the active ingredient were < 0.1 mg Aminol/L (limit of quantification, LOQ). All effect values are given based on the nominal concentrations. Details of the analytical results are given in part 10.

Table 1: NOEC, LOEC and EC50-Values (0-72 h) of AMINOL based on nominal concentrations [mg/L] NOEC and LOEC values were calculated using One Way Analysis of Variance, DUNNETT'S Method and BONFERRONI t-test (a = 0.05)

	Inhibition of biomass growth [mg/L]
EbC50	17.9
95% confidence interval	15.2 -21.2
LOEC	25
NOEC	12.5
	Rate-related Inhibition [mg/L]
ErC50	56.0
95% confidence interval	47.7 -65.7
LOEC	25
NOEC	12.5

After 72 h algae were transferred from the nominal dosage levels 25 - 100 mg/L and control to fresh untreated medium and allowed to grow for further 3 d under test conditions. The test item effect was observed to be reversible at these concentration levels.