2-[2-[4-(diethylamino)phenyl]vinyl]-1,3,3-trimethyl-3H-indolium acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of read across chemicals

Justification for type of information:

Data from handbook or collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE was prepared to determine the effect of test chemical on the mobility of aquatic invertebrates Daphnai magna.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

WoE 2, 3: The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution.

WoE 4:
The stock solution (10 mg/L) was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Hardness:

145.5 mg of CaCO3

Test temperature:

20±2 ° C

pH:

WoE 2: 7.1
WoE 4: Sample at concentration 0.32 mg/L: pH= 8.0 changed to pH= 7.9 during the test
Control: pH= 8.0 changed to pH= 7.9 during the test

Dissolved oxygen:

higher than 8 mg/L at the end of test

Nominal and measured concentrations:

WoE 2, 3: 0.125mg/L, 0.25mg/L, 0.5mg/L,1mg/L, 2mg/L nominal concentrations were used.
WoE 4: 0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

WoE 4:
Details on test conditions
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: WoE - 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.125 - < 0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: WoE - 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.116 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: WoE - 4

Validity criteria fulfilled:

yes

Conclusions:

2: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test Daphnia was determine to be 0.5 mg/L.
3: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia magna was determine to be <0.25 mg/L but >0.125 mg/L.
4: The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study.
Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria.

Executive summary:

Data available for the test chemical has been reviewed to determine the toxicity of test chemical on the mobility of aquatic invertebrates. The studies are as mentioned below:

 

Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia was determine to be 0.5 mg/L. Based on the EC50 value, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria. 

 

Above study was further supported by the study from experimental source. Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test daphnia was determine to be <0.25 mg/L but >0.125 mg/L. Thus based on the EC50 value, it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.

 

Similar study for determination of the inhibition of the mobility of Daphnia magna was carried out with the test substance conducted according to OECD Guideline 202. Test performed under the static system. The test substance was tested at the concentrations 0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L. Effects on immobilisation were observed for 48 hours. EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrate Daphnia magna and can be classified as aquatic acute category 1/ chronic 1 as per the CLP criteria.

 

Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria.

Description of key information

2: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test Daphnia was determine to be 0.5 mg/L.

3: Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia magna was determine to be <0.25 mg/L but >0.125 mg/L.

4: The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study.

Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.5 mg/L

Additional information

Data available for the test chemical and structurally and functionally similar read across chemicals extracted using mechanistic approach has been reviewed to determine the toxicity of test chemical on the mobility of aquatic invertebrates. The studies are as mentioned below:

 

Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on Daphnia was determine to be 0.5 mg/L. Based on the EC50 value, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria. 

 

Above study was further supported by the study from experimental source. Principle of this study was to observe the effect of test chemical on the mobility pattern of aquatic invertebrates. Test conducted in accordance with OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism. The test stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media, further test concentrations 0.125mg/L, 0.25mg/L, 0.5mg/L, 1mg/L, and 2mg/L respectively was prepared from stock solution. 10 Daphnids was added per concentration. Test conducted under the static system for 48 hours. The effect of test chemical was calculated on the basis of immobility observations of Daphnia magna. Observations including (immobility, pH, Temperature, dissolved oxygen content) were recorded in the interval of 24 and 48 hours. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] of test chemical on test daphnia was determine to be <0.25 mg/L but >0.125 mg/L. Thus based on the EC50 value, it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.

 

Similar study for determination of the inhibition of the mobility of Daphnia magna was carried out with the test substance conducted according to OECD Guideline 202. Test performed under the static system. The test substance was tested at the concentrations 0, 0.01, 0.02, 0.04, 0.08, 0.16 and 0.32 mg/L. Effects on immobilisation were observed for 48 hours. EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance on Daphnia magna was determined to be 0.016 mg/L on the basis of mobility inhibition effects in a 48 hour study. This value indicates that the substance is likely to be hazardous to aquatic invertebrate Daphnia magna and can be classified as aquatic acute category 1/ chronic 1 as per the CLP criteria.

 

Under the test condition by observing the effects on the mobility of Daphnia magna, it was consider that the chemical was toxic and can be consider to be classified in aquatic acute 1/chronic 1 as per the CLP classification criteria.