2-amino-2-methylpropane-1,3-diol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 Jan 1997 - 05 Feb 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: EN ISO 10712: 1995

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution in deionised water (12500 mg/L). pH adjusted to 7.2
- after addition of test substance replicates were shaken at 120 rpm and 23 ± 1 °C in the dark
- sterile preparation of replicates

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: strain "Migula, DSM 50026"
- Preparation of inoculum for exposure: concentration of inoculum at test start 50 FNU (Formazin Nephelometric Units), single replicates at test start 5 FNU (1/10 dilution)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test temperature:

23 °C

pH:

7.2

Nominal and measured concentrations:

nominal: 0, 31.5, 100, 312.5, 1000, 3125, and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 mL glass Erlenmeyer flaks; all sterilised before use
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Culture / test medium: the following substances were diluted in water and filled up to 500 mL

solution 1:
NaNO3: 10 g
K2HPO4: 2.4 g
KH2PO4: 1.2 g
yeast extract: 1 g

solution 2:
NaNO3: 10 g
K2HPO4: 2.4 g
KH2PO4: 1.2 g

solution 3:
D(+)-Glucosemonohydrate x H2O: 10 g

solution 4:
MgSO4 x 7 H2O: 4 g
granular iron(III)citrate: 0.01 g

Stock solution: 18 g Agar, 50 mL solution 1, 125 mL solution 3, 100 mL solution 4, 1000 mL water; fluid medium diveded into 6-10 mL portions in culture vials, sealed, and sterilised for 10 min at 121 °C. Afterwards stored at 2 to 4 °C

OTHER TEST CONDITIONS
- Adjustment of pH: yes, 7.2

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : optical density of bacteria suspension was measured at 525 nm with a Merck SQ 118 photometer; measurements at the start and at the end of the test

Reference substance (positive control):

yes

Remarks:

2,3-Dichlorphenol

Key result

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: turbidity

Key result

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: turbidity

Results with reference substance (positive control):

EC50 (16 h) > 10 <30 mg/L (demand value: 10 - 30 mg/L)

Table 1: turbidity measurements and median values (FNUs), standard deviations, variation coefficients, and growth inhibition

		Measured FNUs
	Concentration (mg/L)	Flask 1	Flask 2	Flask 3	Median value	Standard deviation	Variation coefficient	Growth inhibition
Control, 0 h		6			6
Test substance, 16 h	10000	568	570	565	568	2.5	0.4	6.9
	3125	610	630	585	608	22.5	3.7	0.1
	1000	600	610	603	604	5.2	0.9	0.8
	312.5	610	600	608	606	5.2	0.9	0.6
	100	618	613	630	620	9.0	1.5	-1.8
	31.5	623	628	605	618	11.8	1.9	-1.5
Reference substance, 16 h	30	5	5	5	5	0	0	100.2
	10	610	605	618	611	6.292	1.03	-0.3
Control, 16 h		603	615	610	609	6.292	1.033	0

Validity criteria fulfilled:

yes

Key value for chemical safety assessment

EC50 for microorganisms:

10 000 mg/L

EC10 or NOEC for microorganisms:

10 000 mg/L