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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: screening test, other
Modified MITI Test (II), according to OECD Guideline 302C.
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 Jan - 24 Feb 1997
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
according to guideline
OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
GLP compliance:
Oxygen conditions:
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: municipal sewage treatment plant Reutlingen
- Pretreatment: twice washed with dechlorinated tap water and centrifuged; dry weight of centrifuged sludge is 60 mg/g wet sludge
- Concentration of sludge: in the test a concentration of 100 mg/L dry weight is used
Duration of test (contact time):
22 d
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
- Composition of medium: composition according to OECD 302C (6 instead of 3 mL per solution are used)
- Test temperature: 25 ± 1 °C
- pH: 7.0
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: 100 mg/L

- Culturing apparatus:
- Number of culture flasks/concentration: 4
- Measuring equipment: BOD measuring device BSB 1002, company WTW
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: CO2 absorption with KOH

- Inoculum blank: yes, 3 replicates
- Abiotic sterile control: yes, 3 replicates
- Toxicity control: yes, 3 replicates
Reference substance:
benzoic acid, sodium salt
Key result
% degradation (CO2 evolution)
Sampling time:
22 d
Results with reference substance:
Degradation after 22 d: 74.9 %

The BOD (22 d) of the test substance is 61.9 mg O2/L (less blank and abiotic control). The ThOD of the test substance is 64 mg O2/L.

Validity criteria fulfilled:
Interpretation of results:
inherently biodegradable
Degradation of the test substance after 22 d: 96.7 %. The substance is inherently biodegradable in the Modified MITI Test (II), according to OECD Guideline 302C.
Executive summary:

The substance is inherently biodegradable in the Modified MITI Test (II), according to OECD Guideline 302C.

Description of key information

The substance is inherently biodegradable in the Modified MITI Test (II), according to OECD Guideline 302C.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information